Cultivation of Drought-Tolerant and Insect-Resistant Rice Affects Soil Bacterial, but Not Fungal, Abundances and Community Structures.
Cultivation of Drought-Tolerant and Insect-Resistant Rice Affects Soil Bacterial, but Not Fungal, Abundances and Community Structures.
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DOI：
10.3389/fmicb.2018.01390
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发表时间：
2018
影响因子：
5.2
通讯作者：
Tang X
中科院分区：
文献类型：
--
作者：
Li P;Ye S;Liu H;Pan A;Ming F;Tang X
抗旱抗虫水稻品种对土壤微生物组的影响尚不清楚。因此，本研究旨在探讨耐旱抗虫水稻品种汉恢3t栽培对土壤理化性质和细菌、真菌群落组成的影响。在孕穗期收集汉恢3t和常规水稻品种（汉恢3号和中华11号）的3份土壤样品，分别采用qPCR和Illumina MiSeq测序技术对细菌和真菌种群规模和群落结构进行分析。汉汇3t的Bt蛋白浓度显著高于汉汇3号和中华11号，而pH显著低于汉汇3t。汉汇3t与汉汇3号（或中华11号）的细菌种群数量和群落组成差异显著，而真菌群落差异无统计学意义。这些差异表明，汉辉3t培养对细菌群落组成的影响大于对真菌群落的影响。细菌丰度与土壤pH呈正相关，细菌群落结构变化主要受土壤pH和Bt蛋白浓度差异驱动。综上所述，Hanhui3T培养改变了根际细菌群落的丰度和结构，改变了土壤pH和Bt蛋白浓度，而真菌群落则没有这种反应。
The impacts of rice varieties with stacked drought tolerance and insect resistance on soil microbiomes are poorly understood. Hence, the objective of this study was to investigate the effects resulting from the cultivation of the drought-tolerant and insect-resistant rice cultivar, Hanhui3T, on soil physical–chemical properties, and bacterial and fungal community composition. Soil samples of Hanhui3T and conventional rice varieties (Hanhui3 and Zhonghua11) were collected in triplicate at the booting stage, and bacterial and fungal population sizes and community structures were assessed using qPCR and Illumina MiSeq sequencing, respectively. The Bt protein concentration of Hanhui3T was significantly higher than that of Hanhui3 and Zhonghua11, while the pH of Hanhui3T was significantly lower. Bacterial population sizes and community composition were significantly different between Hanhui3T and Hanhui3 (or Zhonghua11), while no similar effects were observed for fungal communities. These differences suggest that the effect of Hanhui3T cultivation on bacterial community composition is stronger than the effect on fungal communities. Moreover, bacterial abundance was positively correlated to soil pH, while bacterial community structure variations were mainly driven by soil pH and Bt protein concentration differences. In conclusion, the abundances and structure of bacterial communities were altered in rhizosphere with Hanhui3T cultivation that changed soil pH and Bt protein concentrations, while fungal communities displayed no such responsiveness.
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影响因子：
4.8
作者：
Hill, GT;Mitkowski, NA;Nelson, EB
通讯作者：
Nelson, EB
影响因子：
11
作者：
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5.1
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