Unveiling the mechanism of uptake and sub-cellular distribution of cerium oxide nanoparticles.
Unveiling the mechanism of uptake and sub-cellular distribution of cerium oxide nanoparticles.
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DOI：
10.1039/c0mb00014k
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发表时间：
2010-10
影响因子：
--
通讯作者：
Self WT
中科院分区：
文献类型：
--
作者：
Singh S;Kumar A;Karakoti A;Seal S;Self WT
氧化铈纳米颗粒（CNP）最近已经研究了其有效的超氧化物清除性能在细胞和动物模型系统。来自这些模型系统的数据表明，细胞暴露于CNP导致针对活性氧（ROS）的保护。尽管有这些令人兴奋的发现，但关于这些纳米材料在细胞内的摄取或亚细胞分布知之甚少。在这项研究中，我们利用荧光团（carboxyfluorescein）共轭氧化铈纳米粒子（CCNPs）的摄取机制，并阐明了使用角质形成细胞模型系统的亚细胞定位的CNPs。我们观察到CCNPs的快速摄取（3小时内），这是由能量依赖性，网格蛋白介导和小窝介导的内吞途径。我们发现CCNPs与线粒体、溶酶体和内质网共定位，并且在细胞质和细胞核中丰富。考虑到氧化铈的自由基清除特性和我们观察到的广泛的细胞分布，CNP可能在细胞的多个隔室中充当细胞抗氧化剂，从而提供针对各种氧化剂损伤的保护。
Cerium oxide nanoparticles (CNPs) have been recently studied for their potent superoxide scavenging properties in both cell and animal model systems. Data from these model systems have shown that exposure of cells to CNPs results in the protection against reactive oxygen species (ROS). Despite these exciting findings, very little is known regarding the uptake or subcellular distribution of these nanomaterials inside cells. In this study we utilized fluorophore (carboxyfluorescein) conjugated cerium oxide NPs (CCNPs) to study the mechanism of uptake and to elucidate the subcellular localization of CNPs using a keratinocyte model system. We observed rapid uptake (within 3 h) of CCNPs that was governed by energy-dependent, clathrin-mediated and caveolae-mediated endocytic pathways. We found CCNPs co-localized with mitochondria, lysosomes and endoplasmic reticulum as well as being abundant in the cytoplasm and the nucleus. Given the radical scavenging properties of cerium oxide and the widespread cellular disposition we observed, CNPs likely act as cellular antioxidants in multiple compartments of the cell imparting protection against a variety of oxidant injuries.
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影响因子：
15
作者：
Kam, NWS;Dai, HJ
通讯作者：
Dai, HJ
影响因子：
3.7
作者：
Karakoti, A. S.;Kuchibhatla, Satyanarayana V. N. T.;Seal, S.
通讯作者：
Seal, S.
影响因子：
15
作者：
Karakoti, Ajay S.;Singh, Sanjay;Kumar, Amit;Malinska, Maura;Kuchibhatla, Satyanarayana V. N. T.;Wozniak, Krzysztof;Self, William T.;Seal, Sudipta
通讯作者：
Seal, Sudipta
影响因子：
46.9
作者：
Jaiswal, JK;Mattoussi, H;Simon, SM
通讯作者：
Simon, SM
影响因子：
14
作者：
Heckert, Eric G.;Karakoti, Ajay S.;Self, William T.
通讯作者：
Self, William T.