Blue light for infectious diseases: Propionibacterium acnes, Helicobacter pylori, and beyond?
Blue light for infectious diseases: Propionibacterium acnes, Helicobacter pylori, and beyond?
复制标题
DOI：
10.1016/j.drup.2012.07.001
复制
发表时间：
2012-08
影响因子：
24.3
通讯作者：
Hamblin, Michael R.
中科院分区：
文献类型：
--
作者：
Dai, Tianhong;Gupta, Asheesh;Murray, Clinton K.;Vrahas, Mark S.;Tegos, George P.;Hamblin, Michael R.
关键词：
蓝光，特别是在405-470 nm波长范围内的蓝光，由于其固有的抗微生物作用而不添加外源性光敏剂，引起了越来越多的关注。此外，人们普遍认为蓝光对哺乳动物细胞的危害比紫外线照射小得多，紫外线照射是另一种正在研究的基于光的抗菌方法。本文就蓝光在微生物细胞中的感受系统、蓝光的抗菌效果、蓝光的抗菌机制、蓝光对哺乳动物细胞的影响以及蓝光对伤口愈合的影响等方面进行了综述。据报道，蓝光可以通过细菌中的蓝光受体调节涉及细胞间通讯的多细胞行为，并抑制生物膜形成，随后增强光灭活。在较高的辐射暴露下，蓝光对革兰氏阳性菌和革兰氏阴性菌都表现出广谱抗菌作用。蓝光疗法是临床上接受的痤疮丙酸杆菌感染的方法。也进行了临床试验来研究使用蓝光治疗幽门螺杆菌胃感染，并显示出有希望的结果。还报道了蓝光灭活重要伤口致病菌的研究，包括金黄色葡萄球菌和铜绿假单胞菌。蓝光灭活痤疮丙酸杆菌、H.幽门螺杆菌和一些口腔细菌的一个重要原因是细胞内卟啉的光激发和随后细胞毒性活性氧的产生。尽管情况可能是伤口病原体的蓝光失活机制（例如，S.金黄色葡萄球菌（P. aureus）、铜绿假单胞菌（P. aeruginosa））与痤疮假单胞菌（P. acnes）相同，但这一假设尚未得到严格检验。关于蓝光对哺乳动物细胞和伤口愈合的影响，报告了有限和不一致的结果。在某些波长和辐射暴露下，蓝光可通过光激发线粒体或/和过氧化物酶体内的蓝光敏感发色团（包括黄素和细胞色素）而引起细胞功能障碍。应该进行进一步的研究以优化光学参数（例如，波长，辐射暴露），以确保有效和安全的蓝光治疗传染病。此外，还需要研究来验证微生物对蓝光的抗性缺乏发展。
Blue light, particularly in the wavelength range of 405–470 nm, has attracted increasing attention due to its intrinsic antimicrobial effect without the addition of exogenous photosensitizers. In addition, it is commonly accepted that blue light is much less detrimental to mammalian cells than ultraviolet irradiation, which is another light-based antimicrobial approach being investigated. In this review, we discussed the blue light sensing systems in microbial cells, antimicrobial efficacy of blue light, the mechanism of antimicrobial effect of blue light, the effects of blue light on mammalian cells, and the effects of blue light on wound healing. It has been reported that blue light can regulate multi-cellular behavior involving cell-to-cell communication via blue light receptors in bacteria, and inhibit biofilm formation and subsequently potentiate light inactivation. At higher radiant exposures, blue light exhibits a broad-spectrum antimicrobial effect against both Gram-positive and Gram-negative bacteria. Blue light therapy is a clinically accepted approach for Propionibacterium acnes infections. Clinical trials have also been conducted to investigate the use of blue light for Helicobacter pylori stomach infections and have shown promising results. Studies on blue light inactivation of important wound pathogenic bacteria, including Staphylococcus aureus and Pseudomonas aeruginosa have also been reported. The mechanism of blue light inactivation of P. acnes, H. pylori, and some oral bacteria is the photo-excitation of intracellular porphyrins and the subsequent production of cytotoxic reactive oxygen species. Although it may be the case that the mechanism of blue light inactivation of wound pathogens (e.g., S. aureus, P. aeruginosa) is the same as that of P. acnes, this hypothesis has not been rigorously tested. Limited and discordant results have been reported regarding the effects of blue light on mammalian cells and wound healing. Under certain wavelengths and radiant exposures, blue light may cause cell dysfunction by the photo-excitation of blue light sensitive chromophores, including flavins and cytochromes, within mitochondria or/and peroxisomes. Further studies should be performed to optimize the optical parameters (e.g., wavelength, radiant exposure) to ensure effective and safe blue light therapies for infectious disease. In addition, studies are also needed to verify the lack of development of microbial resistance to blue light.
登录
查看更多内容
影响因子：
3.3
作者：
Dai, Tianhong;Huang, Ying-Ying;Hamblin, Michael R.
通讯作者：
Hamblin, Michael R.
影响因子：
4.9
作者：
Dai, Tianhong;de Arce, Vida J. Bil;Hamblin, Michael R.
通讯作者：
Hamblin, Michael R.
影响因子：
4.9
作者：
Dai, Tianhong;Garcia, Barbara;Hamblin, Michael R.
通讯作者：
Hamblin, Michael R.
影响因子：
3.3
作者：
Feuerstein, O;Ginsburg, I;Weiss, EI
通讯作者：
Weiss, EI
影响因子：
3.4
作者：
Eady, EA;Gloor, M;Leyden, JJ
通讯作者：
Leyden, JJ