Towards a standardized multi-tissue decellularization protocol for the derivation of extracellular matrix materials.
Towards a standardized multi-tissue decellularization protocol for the derivation of extracellular matrix materials.
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DOI：
10.1039/d2bm01012g
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发表时间：
2023-01-17
影响因子：
6.6
通讯作者：
Freytes, Donald O. O.
中科院分区：
文献类型：
--
作者：
Biehl, Andreea;Martins, Ana M. Gracioso M.;Davis, Zachary G. G.;Sze, Daphne;Collins, Leonard;Mora-Navarro, Camilo;Fisher, Matthew B. B.;Freytes, Donald O. O.
组织脱细胞的目标是有效地去除不需要的细胞成分，如DNA和细胞碎片，同时保留细胞外基质(ECM)内复杂的结构和分子环境。到目前为止，脱细胞方案主要集中在定制的特定组织和特定实验室的方案上，这些方案包括连续的手动步骤，从而产生可变的和特定于方案的ECM材料。脱细胞ECM之间的不一致方案导致的差异会影响批次之间的一致性，限制不同实验室获得的结果之间的比较，并可能限制材料在实验室或临床上一致使用的可转移性。这项研究是开发标准化方案的第一个概念验证，该方案可用于通过先前建立的自动化系统来获得多种ECM生物材料(粉末和水凝胶)。使用我们团队开发的自动去细胞方法是因为其去细胞时间短(4小时)，并且能够减少批次之间的变异性。使用统一方案的第一次迭代获得的细胞外基质能够从皮肤、肺、肌肉、肌腱、软骨和喉组织中产生细胞外基质水凝胶。所有在本研究中形成的水凝胶都是细胞相容的，并且表现出与以前的ECM水凝胶一致的凝胶和流变性。细胞外基质还显示出独特的蛋白质组分。本研究代表了开发标准化方案的第一步，该标准化方案可以快速、可扩展和可重复的方式用于多个组织。这项研究代表了用于ECM生物材料衍生的第一个概念验证标准化的自动多组织去细胞方案。
The goal of tissue decellularization is to efficiently remove unwanted cellular components, such as DNA and cellular debris, while retaining the complex structural and molecular milieu within the extracellular matrix (ECM). Decellularization protocols to date are centered on customized tissue-specific and lab-specific protocols that involve consecutive manual steps which results in variable and protocol-specific ECM material. The differences that result from the inconsistent protocols between decellularized ECMs affect consistency across batches, limit comparisons between results obtained from different laboratories, and could limit the transferability of the material for consistent laboratory or clinical use. The present study is the first proof-of-concept towards the development of a standardized protocol that can be used to derive multiple ECM biomaterials (powders and hydrogels) via a previously established automated system. The automated decellularization method developed by our group was used due to its short decellularization time (4 hours) and its ability to reduce batch-to-batch variability. The ECM obtained using this first iteration of a unified protocol was able to produce ECM hydrogels from skin, lung, muscle, tendons, cartilage, and laryngeal tissues. All hydrogels formed in this study were cytocompatible and showed gelation and rheological properties consistent with previous ECM hydrogels. The ECMs also showed unique proteomic composition. The present study represents the first step towards developing standardized protocols that can be used on multiple tissues in a fast, scalable, and reproducible manner. This study represents the first proof-of-concept standardized automated multi-tissue decellularization protocol for the derivation of ECM biomaterials.
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影响因子：
5.8
作者：
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通讯作者：
Schlesner, Matthias
影响因子：
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作者：
Badileanu A;Mora-Navarro C;Gracioso Martins AM;Garcia ME;Sze D;Ozpinar EW;Gaffney L;Enders JR;Branski RC;Freytes DO
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影响因子：
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通讯作者：
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