Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae.
Functional analysis of ScSwi1 and CaSwi1 in invasive and pseudohyphal growth of Saccharomyces cerevisiae.
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ScSwi1 和 CaSwi1 在酿酒酵母侵入性和假菌丝生长中的功能分析。
DOI：
10.1093/abbs/gmp047
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发表时间：
2009
影响因子：
3.7
通讯作者：
Jiangye Chen
中科院分区：
文献类型：
--
作者：
X. Mao;X. Nie;F. Cao;Jiangye Chen
本文报道，在酿酒酵母中，删除Swi/Snf复合物的核心组分Swi1 （ScSwi1）会导致侵入性生长、假菌丝生长、FLO11表达和细胞正常分离的缺陷。将SWI1重新引入到SWI1突变体中可以抑制所观察到的所有缺陷。我们还发现过量产生Swi1可以抑制flo8细胞在二倍体假菌丝生长中的缺陷，但对单倍体的侵袭性生长没有作用。过表达SWI1不能绕过Ste12或Tec1在侵袭性生长或假菌丝生长中的要求。我们得出结论，FLO11的表达和细胞分离需要Swi/Snf复合体，并且FLO8和STE12基因都需要复合体在侵袭性生长中发挥作用，而假菌丝的生长只需要STE12基因。异位表达的白色念珠菌SWI1 （CaSWI1）可以部分补充单倍体Scswi1突变体所检测的缺陷，但不能补充二倍体Scswi1/ Scswi1突变体所检测的缺陷。过表达CaSwi1减轻了MAP激酶和cAMP通路缺失导致的侵袭性和假菌丝生长缺陷。过表达CaSwi1促进酿酒葡萄球菌的侵袭性和丝状生长需要Swi/Snf复合物的完整性。
Here we reported that, in Saccharomyces cerevisiae, deleting Swi1 (ScSwi1), a core component in Swi/Snf complex, caused defects of invasive growth, pseudohyphal growth, FLO11 expression, and proper cell separation. Re-introduction of SWI1 into the swi1 mutants could suppress all defects observed. We also showed that overproducing Swi1 could suppress the defect of flo8 cells in pseudohyphal growth in diploids, but not invasive growth in haploids. Overexpression of SWI1 could not bypass the requirement of Ste12 or Tec1 in invasive growth or pseudohyphal growth. We concluded that the Swi/Snf complex was required for FLO11 expression and proper cell separation, and both the FLO8 and STE12 genes should be present for the complex to function for the invasive growth but only the STE12 gene was required for the pseudohyphal growth. Ectopic expression of Candida albicans SWI1 (CaSWI1) could partially complement the defects examined of haploid Scswi1 mutants, but failed to complement the defects examined of diploid Scswi1/ Scswi1 mutants. Overexpressing CaSwi1 mitigated invasive and pseudohyphal growth defects resulting from deletions in the MAP kinase and cAMP pathways. The integrity of S. cerevisiae Swi/Snf complex is required for invasive and filamentous growth promoted by overexpressing CaSwi1.
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影响因子：
3.3
作者：
Lu, Yang;Su, Chang;Chen, Jiangye
通讯作者：
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影响因子：
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