Physical Activity Attenuates the Obesity-Induced Dysregulated Expression of Brown Adipokines in Murine Interscapular Brown Adipose Tissue.
Physical Activity Attenuates the Obesity-Induced Dysregulated Expression of Brown Adipokines in Murine Interscapular Brown Adipose Tissue.
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DOI：
10.3390/ijms221910391
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发表时间：
2021-09-27
影响因子：
5.6
通讯作者：
Kizaki T
中科院分区：
文献类型：
--
作者：
Sakurai T;Fukutomi T;Yamamoto S;Nozaki E;Kizaki T
近年来，已证实即使在成人中也存在具有高产热能力的棕色脂肪组织（BAT），并且其已成为预防和改善肥胖和生活方式相关疾病的焦点。然而，肥胖和体力活动（PA）对BAT（棕色脂肪因子）分泌的液体因子的影响还没有很好的了解。因此，在这项研究中，我们专注于棕色脂肪因子，并调查肥胖和PA的影响。在高脂饮食喂养4个月的肥胖小鼠肩胛间BAT中观察到基因流体因子如半乳糖凝集素3（Lgals 3）和Lgals 3结合蛋白（Lgals 3bp）的异常表达，其蛋白质由HB 2棕色脂肪细胞分泌。PA减弱了这些基因表达的异常。此外，尽管与棕色脂肪细胞分化相关的因子（如过氧化物酶体增殖物激活受体γ共激活因子1-α）的基因表达在肥胖小鼠的BAT中也下调，但PA抑制了这些因子的下调。另一方面，与对照细胞相比，过表达Lgals 3的HB 2细胞脂肪生成增加，并且Lgals 3bp的过表达降低了线粒体质量。这些结果表明，PA减弱了肥胖诱导的棕色脂肪因子的表达失调，并表明Lgals 3和Lgals 3bp参与棕色脂肪细胞分化。
In recent years, brown adipose tissue (BAT), which has a high heat-producing capacity, has been confirmed to exist even in adults, and it has become a focal point for the prevention and the improvement of obesity and lifestyle-related diseases. However, the influences of obesity and physical activity (PA) on the fluid factors secreted from BAT (brown adipokines) are not well understood. In this study, therefore, we focused on brown adipokines and investigated the effects of obesity and PA. The abnormal expressions of gene fluid factors such as galectin-3 (Lgals3) and Lgals3 binding protein (Lgals3bp), whose proteins are secreted from HB2 brown adipocytes, were observed in the interscapular BAT of obese mice fed a high-fat diet for 4 months. PA attenuated the abnormalities in the expressions of these genes. Furthermore, although the gene expressions of factors related to brown adipocyte differentiation such as peroxisome proliferator-activated receptor gamma coactivator 1-α were also down-regulated in the BAT of the obese mice, PA suppressed the down-regulation of these factors. On the other hand, lipogenesis was increased more in HB2 cells overexpressing Lgals3 compared with that in control cells, and the overexpression of Lgals3bp decreased the mitochondrial mass. These results indicate that PA attenuates the obesity-induced dysregulated expression of brown adipokines and suggests that Lgals3 and Lgals3bp are involved in brown adipocyte differentiation.
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DOI：
10.1126/science.1190816
发表时间：
2010-05-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Ishibashi J;Seale P
通讯作者：
Seale P
影响因子：
29
作者：
Deshmukh, Atul S.;Peijs, Lone;Scheele, Camilla
通讯作者：
Scheele, Camilla
影响因子：
3
作者：
Grassadonia, A;Tinari, N;Iacobelli, S
通讯作者：
Iacobelli, S
影响因子：
7.7
作者：
Challa, Tenagne D.;Straub, Leon G.;Wolfrum, Christian
通讯作者：
Wolfrum, Christian
DOI：
10.1016/j.bbalip.2018.04.013
发表时间：
2019-01
期刊：
Biochimica et biophysica acta. Molecular and cell biology of lipids
影响因子：
--
作者：
Dewal RS;Stanford KI
通讯作者：
Stanford KI