Comprehensive Cloning of Prunus mume Dormancy Associated MADS-Box Genes and Their Response in Flower Bud Development and Dormancy.
Comprehensive Cloning of Prunus mume Dormancy Associated MADS-Box Genes and Their Response in Flower Bud Development and Dormancy.
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梅花休眠相关MADS-Box基因的综合克隆及其对花芽发育和休眠的响应
DOI：
10.3389/fpls.2018.00017
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Zhao K;Zhou Y;Ahmad S;Xu Z;Li Y;Yang W;Cheng T;Wang J;Zhang Q
休眠相关的MADS-box基因是SVP/MADS-box成员，在多年生植物的休眠中起着至关重要的作用。在梅花中，PmDAM6已被发现可以诱导植物休眠。在目前的研究中，我们克隆了6个PmDAM基因，并在酵母和烟草中进行了功能分析，以检测与PmDAM6相似的基因的作用。表达模式和序列相似性表明，PmDAM在SVP组内分为两个亚支。此外，PmDAM还被证实以某种复杂的模式参与植物不同器官的发育，特别是花蕾的发育。此外，PmDAM蛋白在花芽发育和休眠诱导过程中形成相应的蛋白质复合体，具有特殊的功能。特别是当PmDAM1在暖月份的花蕾中占主导地位时，蛋白质复合体由PmDAM1本身或与PmDAM2组成。在接下来的几个月里，随着温度的降低，PmDAM6得到了高表达，并由于与PmDAM1和PmDAM3强烈的结合倾向，逐渐将复杂的结构转变为PmDAM6-蛋白质复合体。最后，PmDAM6的同源二聚体起到了诱导休眠的作用。目前的研究结果突出了PmDAM在组织发育和休眠中的功能，这为进一步探索与芽生长和休眠相关的蛋白质复合体功能提供了有用的建议。
Dormancy Associated MADS-box genes are SVP/MADs-box members and supposed to play crucial roles in plant dormancy of perennial species. In Prunus mume, PmDAM6 has been previously identified to induce plant dormancy. In the current study, six PmDAMs were cloned in P. mume and functionally analyzed in yeast and tobacco to detect the roles of the genes paralogous to PmDAM6. The expression patterns together with sequence similarities indicate that PmDAMs are divided into two sub-clades within SVP group. Moreover, PmDAMs are verified to take part in the development of different plant organs, specifically the flower buds, in some intricate patterns. Furthermore, the PmDAM proteins are found to have special functions by forming corresponding protein complex during the development of flower bud and induction of dormancy. In particular, when PmDAM1 dominating in flower bud in the warm months, the protein complexes are consisted of PmDAM1 itself or with PmDAM2. With the decrease temperatures in the following months, PmDAM6 was found to be highly expressed and gradually changed the complex structure to PmDAM6-protein complex due to strong binding tendencies with PmDAM1 and PmDAM3. Finally, the homodimers of PmDAM6 prevailed to induce the dormancy. The results obtained in the current study highlight the functions of PmDAMs in the tissue development and dormancy, which provide available suggestions for further explorations of protein-complex functions in association with bud growth and dormancy.
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影响因子：
11.6
作者：
Ruttink, Tom;Arend, Matthias;Rohde, Antje
通讯作者：
Rohde, Antje
综合转录组分析揭示了茶树腋芽在旁休眠、内休眠、生态休眠和芽盛期的差异基因表达谱
影响因子：
5.6
作者：
Hao X;Yang Y;Yue C;Wang L;Horvath DP;Wang X
通讯作者：
Wang X
大米中的MADS-box基因家族：在生殖发育和压力期间，全基因组识别，组织和表达分析。
影响因子：
4.4
作者：
Arora, Rita;Agarwal, Pinky;Ray, Swatismita;Singh, Ashok Kumar;Singh, Vijay Pal;Tyagi, Akhilesh K.;Kapoor, Sanjay
通讯作者：
Kapoor, Sanjay
影响因子：
7.4
作者：
Diaz-Riquelme, Jose;Lijavetzky, Diego;Jose Carmona, Maria
通讯作者：
Jose Carmona, Maria
影响因子：
5.1
作者：
Horvath, David P.;Sung, Sibum;Anderson, James
通讯作者：
Anderson, James