Identification of cotton MOTHER OF FT AND TFL1 homologs, GhMFT1 and GhMFT2, involved in seed germination
Identification of cotton MOTHER OF FT AND TFL1 homologs, GhMFT1 and GhMFT2, involved in seed germination
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鉴定参与种子萌发的棉花 MOTHER OF FT 和 TFL1 同源物 GhMFT1 和 GhMFT2
DOI：
10.1371/journal.pone.0215771
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发表时间：
2019-04
期刊：
影响因子：
3.7
通讯作者：
Xianzhong Huang
中科院分区：
文献类型：
--
作者：
Xiuli Yu;Hui Liu;Na Sang;Yunfei Li;Tingting Zhang;Jie Sun;Xianzhong Huang
植物磷脂酰乙醇胺结合蛋白（PEBP）由3个进化枝组成：FLOWARLOCUS T（FT）、MATRIX FLOWER 1（TFL 1）和MOTHER OF FT AND TFL 1（MFT）。FT/TFL 1-like进化枝调节有限和无限分生组织的身份，并最终影响开花时间和植物结构。MFT通常被认为是FT/TFL 1的祖先，但其功能尚不清楚。本研究通过全基因组范围内的MFT类基因鉴定，在陆地棉中发现了两对MFT同源基因，即GhMFT 1-A/D和GhMFT 2-A/D。详细的表达分析表明，GhMFT 1和GhMFT 2同源基因主要在胚珠中表达，在胚珠发育过程中表达量显著增加，而在种子萌发过程中表达量迅速下降。发芽种子中GhMFT 1和GhMFT 2同源异型基因的表达受阿坝的诱导而上调，它们的表达也受GA的诱导。此外，拟南芥中GhMFT 1和GhMFT 2的异位过表达抑制了早期种子萌发。基因转录分析表明，阿坝代谢基因ABA-INSENSITIVE 3（ABI 3）和ABI 5，GA信号转导途径基因REDUOR OF ga 1 -3（RGA）和RGA-LIKE 2（RGL 2）在35 S：GhMFT 1和35 S：GhMFT 2转基因拟南芥种子中表达上调。GhMFT 1和GhMFT 2定位于细胞质和细胞核中，均与棉花bZIP转录因子GhFD相互作用，表明GhMFT 1和GhMFT 2具有相似的细胞内调控机制。两者合计，结果表明，GhMFT 1和GhMFT 2可能在种子萌发的调节中起冗余和差异的作用。
Plant phosphatidylethanolamine-binding protein (PEBP) is comprised of three clades: FLOWERING LOCUS T (FT), TERMINAL FLOWER1 (TFL1) and MOTHER OF FT AND TFL1 (MFT). FT/TFL1-like clades regulate identities of the determinate and indeterminate meristems, and ultimately affect flowering time and plant architecture. MFT is generally considered to be the ancestor of FT/TFL1, but its function is not well understood. Here, two MFT homoeologous gene pairs in Gossypium hirsutum, GhMFT1-A/D and GhMFT2-A/D, were identified by genome-wide identification of MFT-like genes. Detailed expression analysis revealed that GhMFT1 and GhMFT2 homoeologous genes were predominately expressed in ovules, and their expression increased remarkably during ovule development but decreased quickly during seed germination. Expressions of GhMFT1 and GhMFT2 homoeologous genes in germinating seeds were upregulated in response to abscisic acid (ABA), and their expressions also responded to gibberellin (GA). In addition, ectopic overexpression of GhMFT1 and GhMFT2 in Arabidopsis inhibited seed germination at the early stage. Gene transcription analysis showed that ABA metabolism genes ABA-INSENSITIVE3 (ABI3) and ABI5, GA signal transduction pathway genes REPRESSOR OF ga1-3 (RGA) and RGA-LIKE2 (RGL2) were all upregulated in the 35S:GhMFT1 and 35S:GhMFT2 transgenic Arabidopsis seeds. GhMFT1 and GhMFT2 localize in the cytoplasm and nucleus, and both interact with a cotton bZIP transcription factor GhFD, suggesting that both of GhMFT1, 2 have similar intracellular regulation mechanisms. Taken together, the results suggest that GhMFT1 and GhMFT2 may act redundantly and differentially in the regulation of seed germination.
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