Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection.
Phenylalanine ammonia-lyase2.1 contributes to the soybean response towards Phytophthora sojae infection.
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苯丙氨酸解氨酶2.1有助于大豆对大豆疫霉感染的反应
DOI：
10.1038/s41598-017-07832-2
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发表时间：
2017-08-03
影响因子：
4.6
通讯作者：
Zhang S
中科院分区：
文献类型：
--
作者：
Zhang C;Wang X;Zhang F;Dong L;Wu J;Cheng Q;Qi D;Yan X;Jiang L;Fan S;Li N;Li D;Xu P;Zhang S
大豆疫霉根茎腐病[甘氨酸max(L.)]稳定。大豆疫霉是一种世界性的破坏性疾病。苯丙氨酸解氨酶（PAL）是研究最广泛的与植物对生物和非生物胁迫反应有关的酶之一。然而，大豆PAL响应toP的分子机制尚不清楚。sojaei在很大程度上是不清楚的。在这里，我们描述了大豆PAL基因家族的一个新成员，GmPAL2.1，它显著诱导大豆蛋白酶。过表达和RNA干扰分析表明GmPAL2.1增强了抗性。转基因大豆。此外，mpal2.1 - ox转基因大豆在p侵染后PAL活性显著高于非转基因植株。而inGmPAL2.1-RNAi大豆植株则较低。进一步分析表明，mpal2.1 - ox转基因大豆的大豆黄酮、染料木素和水杨酸（SA）含量以及甘油的相对含量均显著升高。综上所述，这些结果表明GmPAL2.1在大豆对toP的反应中起着重要的正调节作用。可能是通过增加甘油、大豆黄酮、染料木素和SA的含量。
Phytophthora root and stem rot of soybean [Glycine max(L.) Merr.] caused byPhytophthora sojaeis a destructive disease worldwide. Phenylalanine ammonia-lyase (PAL) is one of the most extensively studied enzymes related to plant responses to biotic and abiotic stresses. However, the molecular mechanism of PAL in soybean in response toP.sojaeis largely unclear. Here, we characterize a novel member of the soybean PAL gene family,GmPAL2.1, which is significantly induced byP.sojae. Overexpression and RNA interference analysis demonstrates that GmPAL2.1 enhances resistance toP.sojaein transgenic soybean plants. In addition, the PAL activity inGmPAL2.1-OX transgenic soybean is significantly higher than that of non-transgenic plants after infection withP.sojae, while that inGmPAL2.1-RNAi soybean plants is lower. Further analyses show that the daidzein, genistein and salicylic acid (SA) levels and the relative content of glyceollins are markedly increased inGmPAL2.1-OX transgenic soybean. Taken together, these results suggest the important role of GmPAL2.1 functioning as a positive regulator in the soybean response toP.sojaeinfection, possibly by enhancing the content of glyceollins, daidzein, genistein and SA.
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影响因子：
3.7
作者：
Dhakal R;Park E;Lee SW;Baek KH
通讯作者：
Baek KH
DOI：
10.3109/10425179109020788
发表时间：
1991-01-01
期刊：
DNA sequence : the journal of DNA sequencing and mapping
影响因子：
--
作者：
Frank, R L;Vodkin, L O
通讯作者：
Vodkin, L O
影响因子：
5.6
作者：
Cheng Q;Li N;Dong L;Zhang D;Fan S;Jiang L;Wang X;Xu P;Zhang S
通讯作者：
Zhang S
影响因子：
3.8
作者：
BURDEN, RS;BAILEY, JA
通讯作者：
BAILEY, JA
影响因子：
6.1
作者：
Boué, SM;Carter, CH;Cleveland, TE
通讯作者：
Cleveland, TE