Measuring differences in compositional properties of bone tissue by confocal Raman spectroscopy.
Measuring differences in compositional properties of bone tissue by confocal Raman spectroscopy.
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DOI：
10.1007/s00223-011-9497-x
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发表时间：
2011-08
影响因子：
4.2
通讯作者：
Mahadevan-Jansen, Anita
中科院分区：
文献类型：
--
作者：
Nyman, Jeffry S.;Makowski, Alexander J.;Patil, Chetan A.;Masui, T. Philip;O'Quinn, Elizabeth C.;Bi, Xiaohong;Guelcher, Scott A.;Nicollela, Daniel P.;Mahadevan-Jansen, Anita
全面的骨折风险决定因素来自组成骨组织的每个等级级别。拉曼光谱是一种能够在近亚微米尺度上表征胶原和矿物相的工具，但拉曼光谱区分骨的成分差异的能力还没有很好的定义。因此，我们分析了多个拉曼峰强度和峰比，以表征它们区分皮质骨内典型的矿化程度较低的骨组织和矿化程度较高的间质组织的能力。为了进一步评估差异的来源，我们收集了嵌入的样品和两个方向的切割的拉曼光谱。每个标本的拉曼峰强度或比值在5个骨组织和5个邻近间质组织内的多个部位之间平均。从骨组织到间质组织的组成中，磷酸磷酸酶(ν-1磷酸，PO_4)与脯氨酸或酰胺Ⅲ的比值最高，分别增加了15.4%和12.5%。每种组织的变异系数都小于5%，而传统的∼1PO4/νI的变异系数为8%，这一峰值比率在每种组织类型的横向和纵向切割之间变化最大。虽然包埋影响了拉曼峰，但它并没有掩盖两种组织类型之间与矿化相关的大多数峰比例的差异。在样本量有限但每个样本有足够数量的拉曼光谱进行空间平均的研究中，ν1PO4/酰胺III或ν1PO4/Pro是最有可能检测到试验组之间组成差异的拉曼特性。
The full range of fracture risk determinants arise from each hierarchical level comprising the organization of bone. Raman spectroscopy is one tool capable of characterizing the collagen and mineral phases at a near sub-micron length scale, but the ability of Raman spectra to distinguish compositional differences of bone is not well defined. Therefore, we analyzed multiple Raman peak intensities and peak ratios to characterize their ability to distinguish between the typically less mineralized osteonal tissue and the more mineralized interstitial tissue in intra-cortical human bone. To further assess origins of variance, we collected Raman spectra from embedded specimens and for 2 orientations of cut. Per specimen, Raman peak intensities or ratios were averaged among multiple sites within 5 osteons and 5 neighboring interstitial tissue. The peak ratios of ν1 phosphate (PO4) to Proline or Amide III detected the highest increases of 15.4% or 12.5%, respectively, in composition from osteonal to interstitial tissue. The coefficient of variance (COV) was less than 5% for each as opposed to a COV of ∼8% for the traditional ν1PO4/Amide I, a peak ratio that varied the most between transverse and longitudinal cuts for each tissue type. Although embedding affected Raman peaks, it did not obscure differences in most peak ratios related to mineralization between the 2 tissue types. In studies with limited sample size but sufficient number of Raman spectra per specimen for spatial averaging, ν1PO4/Amide III or ν1PO4/Proline is the Raman property that is most likely to detect a compositional difference between experimental groups.
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影响因子：
2.4
作者：
Bi, Xiaohong;Patil, Chetan A.;Nyman, Jeffry S.
通讯作者：
Nyman, Jeffry S.
影响因子：
2.4
作者：
Hofmann, Tobias;Heyroth, Frank;Raum, Kay
通讯作者：
Raum, Kay
DOI：
10.1007/978-3-642-02649-2_14
发表时间：
2010-01-01
期刊：
EMERGING RAMAN APPLICATIONS AND TECHNIQUES IN BIOMEDICAL AND PHARMACEUTICAL FIELDS
影响因子：
--
作者：
Morris, Michael D.
通讯作者：
Morris, Michael D.
影响因子：
4.1
作者：
Kazanci, M.;Wagner, H. D.;Fratzl, P.
通讯作者：
Fratzl, P.
影响因子：
6.2
作者：
Gourion-Arsiquaud, Samuel;Burket, Jayme C.;Boskey, Adele L.
通讯作者：
Boskey, Adele L.