A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.
A preliminary study of viral metagenomics of French bat species in contact with humans: identification of new mammalian viruses.
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DOI：
10.1371/journal.pone.0087194
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bourhy H
中科院分区：
文献类型：
--
作者：
Dacheux L;Cervantes-Gonzalez M;Guigon G;Thiberge JM;Vandenbogaert M;Maufrais C;Caro V;Bourhy H
病毒性人畜共患病流行的预测已成为一个重大的公共卫生问题。深入了解作为宿主的关键动物物种中的病毒种群是实现这一目标的重要一步。蝙蝠携带多种病毒，其中一些病毒特别令人感兴趣，因为它们会导致严重的人类疾病。然而，人们对蝙蝠中发现的全球病毒种群（病毒组）的多样性知之甚少。我们确定了与人类密切接触的五种不同的法国食虫蝙蝠物种（共九个标本）的病毒多样性。在合并的组织（脑、肝和肺）上使用序列独立扩增、使用Illumina技术的高通量测序和专用生物信息学分析管道。重叠群和未组装读段序列的比较为每个样本提供了病毒相关序列的全球分类学分布，突出了蝙蝠物种内部和之间的差异。这些病毒组中存在许多病毒家族，包括已知感染细菌、植物/真菌、昆虫或脊椎动物的病毒，最相关的是感染哺乳动物的病毒（逆转录病毒科、疱疹病毒科、布尼亚病毒科、痘病毒科、黄病毒科、呼肠孤病毒科、博尔纳韦病毒科、Picobirnaviridae）。特别是，我们发现了几种新的哺乳动物病毒，包括轮状病毒，γ逆转录病毒，博尔纳病毒和布尼亚病毒与第一蝙蝠内罗病毒的鉴定。这些观察结果表明，蝙蝠自然携带来自许多不同家族的病毒，其中大多数感染哺乳动物。因此，它们可能构成病毒多样性的主要储存库，应仔细分析，以确定蝙蝠在人畜共患病毒感染传播中所起的作用。
The prediction of viral zoonosis epidemics has become a major public health issue. A profound understanding of the viral population in key animal species acting as reservoirs represents an important step towards this goal. Bats harbor diverse viruses, some of which are of particular interest because they cause severe human diseases. However, little is known about the diversity of the global population of viruses found in bats (virome). We determined the viral diversity of five different French insectivorous bat species (nine specimens in total) in close contact with humans. Sequence-independent amplification, high-throughput sequencing with Illumina technology and a dedicated bioinformatics analysis pipeline were used on pooled tissues (brain, liver and lungs). Comparisons of the sequences of contigs and unassembled reads provided a global taxonomic distribution of virus-related sequences for each sample, highlighting differences both within and between bat species. Many viral families were present in these viromes, including viruses known to infect bacteria, plants/fungi, insects or vertebrates, the most relevant being those infecting mammals (Retroviridae, Herpesviridae, Bunyaviridae, Poxviridae, Flaviviridae, Reoviridae, Bornaviridae, Picobirnaviridae). In particular, we detected several new mammalian viruses, including rotaviruses, gammaretroviruses, bornaviruses and bunyaviruses with the identification of the first bat nairovirus. These observations demonstrate that bats naturally harbor viruses from many different families, most of which infect mammals. They may therefore constitute a major reservoir of viral diversity that should be analyzed carefully, to determine the role played by bats in the spread of zoonotic viral infections.
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影响因子：
3.8
作者：
Cui, Jie;Tachedjian, Gilda;Wang, Lin-Fa
通讯作者：
Wang, Lin-Fa
影响因子：
16.6
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影响因子：
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作者：
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通讯作者：
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影响因子：
11.8
作者：
Esona MD;Mijatovic-Rustempasic S;Conrardy C;Tong S;Kuzmin IV;Agwanda B;Breiman RF;Banyai K;Niezgoda M;Rupprecht CE;Gentsch JR;Bowen MD
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影响因子：
5.4
作者：
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通讯作者：
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