Epigenetic Programming by Maternal Behavior in the Human Infant.
Epigenetic Programming by Maternal Behavior in the Human Infant.
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孕产妇行为在人类婴儿中的表观遗传编程。
DOI：
10.1542/peds.2017-1890
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发表时间：
2018-10
期刊：
影响因子：
8
通讯作者：
Marsit CJ
中科院分区：
文献类型：
--
作者：
Lester BM;Conradt E;LaGasse LL;Tronick EZ;Padbury JF;Marsit CJ
我们试图确定孕产妇护理的变化是否会改变足月健康五个月大婴儿的 DNA 甲基化。这项工作基于对动物模型的里程碑式研究，表明母鼠的养育护理会通过表观遗传机制改变新生儿的应激反应。我们用母乳喂养作为动物母性行为的代表。我们假设母乳喂养的母亲所生的婴儿与非母乳喂养的母亲所生的婴儿相比，糖皮质激素受体基因的 DNA 甲基化发生了改变，并且下丘脑应激反应较少。本分析采用了一项队列研究，对象为足月健康婴儿及其前 5 个月母乳喂养 (n=21) 或不母乳喂养 (n=21) 的母亲。使用母婴互动程序和糖皮质激素受体基因重要调节区域的 DNA 甲基化来测量婴儿唾液中的皮质醇应激反应性。将人类该基因的 DNA 甲基化变化与大鼠基因的同源区域进行比较。 DNA 样本是从脸颊拭子中制备的，并使用灵敏的测序技术对甲基化程度进行定量分析。母乳喂养降低了 5 个月大婴儿糖皮质激素受体启动子的 DNA 甲基化，并降低了皮质醇反应性。 DNA 甲基化减少发生在参与调节下丘脑-垂体-肾上腺 (HPA) 和免疫系统反应的启动子区域。人类的孕产妇护理可能会通过行为编程影响 HPA 应激反应，并表现为后代表观遗传变化。这些结果部分解释了在母乳喂养儿童中观察到的一些积极影响。人类对啮齿动物的复制将孕产妇护理与表观遗传变异联系起来，并减少了婴儿压力，从而对母乳喂养的积极影响产生了影响。
We sought to determine if variations in maternal care alter DNA methylation in term, healthy five- month-old infants. This work was based on landmark studies in animal models demonstrating that nurturing care by dams would alter their newborn’s stress responses through epigenetic mechanisms. We used breastfeeding as a proxy for animal maternal behavior. We hypothesized alterations in DNA methylation of the glucocorticoid receptor gene and less hypothalamic stress response in infants of mothers who breastfed their infants versus infants of mothers who did not breastfeed. A cohort study of term, healthy infants and their mothers who did (n=21) or did not (n=21) breastfeed for the first 5 months was utilized in this analysis. Cortisol stress reactivity was measured in infant saliva using a mother-infant interaction procedure, and DNA methylation of an important regulatory region of the glucocorticoid receptor gene. Changes in DNA methylation of this gene in the human were compared to homologous regions of the rat gene. DNA samples were prepared from cheek swabs and subjected to quantitative analysis of extent of methylation using sensitive sequencing techniques. Breastfeeding decreased DNA methylation of the glucocorticoid receptor promoter and decreased cortisol reactivity in 5-month-old infants. Decreased DNA methylation occurred in the promoter region involved in regulation of the hypothalamic-pituitary-adrenal (HPA) and immune system responses. Maternal care in humans may impact HPA stress response through behavioral programming and manifest as offspring epigenetic change. These results explain, in part, some of the positive effects observed in children with breastfeeding. A human replication of rodent work relates maternal care to epigenetic variation and reduced infant stress with implications for the positive effects of breastfeeding.
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影响因子：
3.7
作者：
Conradt, Elisabeth;Lester, Barry M.;Marsit, Carmen J.
通讯作者：
Marsit, Carmen J.
影响因子：
3.7
作者：
Oberlander, Tim F.;Weinberg, Joanne;Devlin, Angela M.
通讯作者：
Devlin, Angela M.
影响因子：
3.8
作者：
Lester, Barry M.;Marsit, Carmen J.;Padbury, James F.
通讯作者：
Padbury, James F.
影响因子：
2.2
作者：
Bromer, Cailey;Marsit, Carmen J.;Armstrong, David A.;Padbury, James F.;Lester, Barry
通讯作者：
Lester, Barry
影响因子：
6.8
作者：
Radtke KM;Ruf M;Gunter HM;Dohrmann K;Schauer M;Meyer A;Elbert T
通讯作者：
Elbert T