Characterization of the osteogenic potential of mesenchymal stem cells from human periodontal ligament based on cell surface markers.
Characterization of the osteogenic potential of mesenchymal stem cells from human periodontal ligament based on cell surface markers.
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DOI：
10.1038/ijos.2015.42
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发表时间：
2015-12-18
影响因子：
14.9
通讯作者：
Hong, Christine
中科院分区：
文献类型：
--
作者：
Alvarez, Ruth;Lee, Hye-Lim;Wang, Cun-Yu;Hong, Christine
间充质干细胞(MSC)介导的治疗已被证明在临床上再生组织缺损是有效的。为了改进再生治疗，分离出具有高分化能力的同源MSCs群体是至关重要的。干细胞表面抗原的利用为从不同组织中鉴定MSCs提供了一种手段。然而，几乎没有持续分离高度再生的MSCs的表面标志物被验证，这使得它对常规的临床应用具有挑战性，并且使得识别可靠的表面标志物变得更加迫切。在这项研究中，我们使用三种表面标记组合：CD51/CD140α、CD271和Stro-1/CD146从异种牙周韧带细胞(PDLC)中分离出均一群的牙间充质干细胞(DMSC)。荧光激活细胞分选分析显示，24%的PDLC为CD51+/CD140α+，0.8%为CD271+，2.4%为Stro-1+/CD146+。通过诱导成骨和成软骨分化，进一步评估了分选细胞群的多潜能特性。所有三个分离的DMSCs亚群都显示出向成骨和成软骨方向分化的能力，但分化程度不同。CD271+DMSCs在DLX5、RUNX2和BGLAP等成骨标志物的强烈诱导下显示出最大的成骨潜能。我们的研究提供了证据，表明本研究中使用的表面标记组合是从PDLC中分离DMSCs的足够标记。这些结果为使用特定的表面标记来识别同质的DMSCs群体以改进其在再生医学中的利用提供了重要的见解。
Mesenchymal stem cell (MSC)-mediated therapy has been shown to be clinically effective in regenerating tissue defects. For improved regenerative therapy, it is critical to isolate homogenous populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, we used three surface marker combinations: CD51/CD140α, CD271, and STRO-1/CD146 for the isolation of homogenous populations of dental mesenchymal stem cells (DMSCs) from heterogeneous periodontal ligament cells (PDLCs). Fluorescence-activated cell sorting analysis revealed that 24% of PDLCs were CD51+/CD140α+, 0.8% were CD271+, and 2.4% were STRO-1+/CD146+. Sorted cell populations were further assessed for their multipotent properties by inducing osteogenic and chondrogenic differentiation. All three subsets of isolated DMSCs exhibited differentiation capacity into osteogenic and chondrogenic lineages but with varying degrees. CD271+ DMSCs demonstrated the greatest osteogenic potential with strong induction of osteogenic markers such as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine.
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影响因子：
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通讯作者：
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作者：
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