Shock wave induced kidney injury promotes calcium oxalate deposition.
Shock wave induced kidney injury promotes calcium oxalate deposition.
复制标题
冲击波引起的肾损伤促进草酸钙沉积。
DOI：
10.1016/j.juro.2009.03.080
复制
发表时间：
2009-08
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
目的体外冲击波碎石术是上尿路肾结石的首选治疗方法。然而，这种治疗与肾结石复发率较高有关。冲击波治疗可导致肾上皮细胞损伤，而肾上皮细胞损伤又是结石形成的最重要因素。我们研究了冲击波继发的肾损伤对肾内草酸钙晶体滞留的影响。材料与方法将32只大鼠随机分为4组，其中组1-对照组，组2-假治疗组大鼠每天给予25 ml 0.75%乙二醇，持续14天，组3-大鼠左肾给予15 kV 1Hz冲击波500次，随后给予25 ml每天0.75%乙二醇，持续14天，第4组——大鼠与第3组进行相同的治疗，只是撞击次数增加到1,000只。实验结束时取出2个肾脏。通过手术显微镜在用苏木精和伊红染色的肾脏切片中观察到草酸钙晶体。使用偏光显微镜测定晶体形态。通过比色法 (Sigma®) 检查酸化的肾组织匀浆中的 Ca 和草酸盐含量。结果肾脏切片显示，未接受冲击波的肾脏比接受冲击波的肾脏具有更少的晶体，而接受冲击波的肾脏在主要区域具有晶体。在第2至4组的左肾中，Ca的平均±SD含量为16.88±6.41、28.58±7.54和40.81±15.29μmol/gm湿肾，草酸盐的平均含量为8.44±6.80、20.52±7.70、31.76±14.14μmol/gm分别为湿肾。草酸钙密度随着冲击波冲击次数的增加而增加。结论冲击波治疗引起的肾损伤可增加该结石模型大鼠肾脏中草酸钙晶体的滞留。
PurposeExtracorporeal shock wave lithotripsy is the preferred treatment for upper urinary tract renal calculi. However, this treatment is associated with a high rate of recurrent renal calculi. Shock wave therapy can result in renal epithelial cell injury, which in turn is a most important factor in calculus formation. We investigated the influence of kidney damage secondary to shock waves on Ca oxalate crystal retention in the kidney.Materials and MethodsA total of 32 rats were randomly divided into 4 groups, including group 1—controls, group 2—sham treated rats given 25 ml 0.75% ethylene glycol per day for 14 days, group 3—rats given 15 kV 1Hz shock waves 500 times to the left kidney, followed by 25 ml 0.75% ethylene glycol daily for 14 days, and group 4—rats with the same treatment as group 3 except the number of impacts was increased to 1,000. The 2 kidneys were removed at the end of the experiment. Ca oxalate crystals were observed by surgical microscopy in kidney sections stained with hematoxylin and eosin. Crystal morphology was determined using polarizing microscopy. Acidified kidney tissue homogenate was examined for Ca and oxalate content by colorimetry (Sigma®).ResultsKidney sections showed that kidneys that did not receive shock waves had fewer crystals than kidneys with shock waves, which had crystals in major areas. In the left kidney in groups 2 to 4 the mean ± SD quantity of Ca was 16.88 ± 6.41, 28.58 ± 7.54 and 40.81 ± 15.29 μmol/gm wet kidney and the mean quantity of oxalate was 8.44 ± 6.80, 20.52 ± 7.70, 31.76 ± 14.14 μmol/gm wet kidney, respectively. Ca oxalate density increased with the number of shock wave impacts.ConclusionsKidney damage caused by shock wave treatment can increase Ca oxalate crystal retention in the kidneys of rats in this stone model.
登录
查看更多内容
影响因子：
2.1
作者：
K. Sarıca;G. Ozer;T. Soygür;O. Yaman;E. Ozer;H. Ustün;L. Yaman;O. Göǧüs
通讯作者：
K. Sarıca;G. Ozer;T. Soygür;O. Yaman;E. Ozer;H. Ustün;L. Yaman;O. Göǧüs
DOI：
10.1016/s0022-5347(01)66127-5
发表时间：
1996-05
期刊：
The Journal of urology
影响因子：
--
作者：
Lesley K. Carr;J. Honey;Michael A.S. Jewett;D. Ibañez;Michele R. Ryan;Claire Bombardier
通讯作者：
Lesley K. Carr;J. Honey;Michael A.S. Jewett;D. Ibañez;Michele R. Ryan;Claire Bombardier
DOI：
10.1016/s0022-5347(01)66535-2
发表时间：
1996
期刊：
The Journal of urology
影响因子：
--
作者：
W. L. Strohmaier;Anemone M. Carl;D. Wilbert;K. Bichler
通讯作者：
W. L. Strohmaier;Anemone M. Carl;D. Wilbert;K. Bichler
影响因子：
3.3
作者：
K. Weinberg;M. Ortiz
通讯作者：
K. Weinberg;M. Ortiz
影响因子：
--
作者：
T. A. Serel;Fehmi Özguner;S. Soyupek
通讯作者：
T. A. Serel;Fehmi Özguner;S. Soyupek