Maternal and paternal genomes contribute equally to the transcriptome of early plant embryos.
Maternal and paternal genomes contribute equally to the transcriptome of early plant embryos.
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DOI：
10.1038/nature10756
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发表时间：
2012-01-22
期刊：
影响因子：
64.8
通讯作者：
Bartel, David P.
中科院分区：
文献类型：
--
作者：
Nodine, Michael D.;Bartel, David P.
在动物中，沉积在卵中的母体基因产物在合子基因组激活之前调节胚胎发育。在植物中，根据基因表达研究，认为存在类似的母体对胚胎发生的长时间控制的时期。然而，其他基因表达研究和遗传分析表明，一些转录本必须源自早期合子基因组，这意味着流行的模型并不能完全解释植物中合子基因组激活的本质。为了确定母本、父本和合子对早期胚胎转录组的贡献，我们对拟南芥两个多态性自交系之间杂交的杂交胚胎的转录本进行了测序，并使用每个亲本系的单核苷酸多态性（SNP）诊断来量化亲本的贡献。尽管一些转录本似乎主要是从父母之一遗传的，或者是从印记基因座转录的，但绝大多数转录本是由母本和父本等位基因以几乎相等的量产生的，即使在胚胎发生的初始阶段也是如此。记者实验和对精子和卵子中不表达的基因转录物的分析结果表明早期和广泛的合子转录。因此，与早期动物胚胎发生相反，早期植物胚胎发生主要受合子控制。
In animals, maternal gene products deposited into eggs regulate embryonic development before activation of the zygotic genome. In plants, an analogous period of prolonged maternal control over embryogenesis is thought to occur based on gene-expression studies. However, other gene-expression studies and genetic analyses show that some transcripts must derive from the early zygotic genome, implying that the prevailing model does not fully explain the nature of zygotic genome activation in plants. To determine the maternal, paternal and zygotic contributions to the early embryonic transcriptome, we sequenced the transcripts of hybrid embryos from crosses between two polymorphic inbred lines of Arabidopsis thaliana and used single-nucleotide polymorphisms (SNPs) diagnostic of each parental line to quantify parental contributions. Although some transcripts appeared to be either inherited from primarily one parent or transcribed from imprinted loci, the vast majority of transcripts were produced in near-equal amounts from both maternal and paternal alleles, even during the initial stages of embryogenesis. Results of reporter experiments and analyses of transcripts from genes that are not expressed in sperm and egg indicate early and widespread zygotic transcription. Thus, in contrast to early animal embryogenesis, early plant embryogenesis is mostly under zygotic control.
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