Creation of male-sterile lines that can be restored to fertility by exogenous methyl jasmonate for the establishment of a two-line system for the hybrid production of rice (Oryza sativa L.).
Creation of male-sterile lines that can be restored to fertility by exogenous methyl jasmonate for the establishment of a two-line system for the hybrid production of rice (Oryza sativa L.).
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创建可通过外源茉莉酸甲酯恢复育性的雄性不育系，用于建立水稻杂交生产的两系系统（Oryza sativa L.）
DOI：
10.1111/pbi.13471
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发表时间：
2021-03
影响因子：
13.8
通讯作者：
Jiang L
中科院分区：
文献类型：
--
作者：
Pak H;Wang H;Kim Y;Song U;Tu M;Wu D;Jiang L
雄性不育被广泛用于水稻杂交种子的生产，但由于维持雄性不育系的劳动力成本高，基因雄性不育的使用受到限制。先前使用T-DNA插入突变的研究表明，破坏参与茉莉酸生物合成途径的氧代植物二烯酸还原酶3（OPR 3）的表达，导致一种雄性不育，可以通过外源茉莉酸在拟南芥中恢复生育力。在这里，我们通过CRISPR/CRISPR相关系统9编辑水稻OsOPR 7的第二和第四外显子来创建雄性不育突变。在这些外显子处的诱变分别导致T0代中31.8%和23.9%的雄性不育植株。我们在T0、T1和T2水稻群体中筛选了可以通过外源茉莉酸甲酯恢复育性的雄性不育系，并表征了转基因植物的花药和农艺性状。结果表明，通过沉默拟南芥OPR 3的直向同源基因OsOPR 7，在大田作物中成功地产生了雄性不育系，为建立用于水稻杂交种生产的双系体系奠定了基础。该系统由可以通过喷洒茉莉酸甲酯保持的雄性不育系和赋予花粉的恢复系组成。
Male sterility is widely used in the production of hybrid seeds in rice, but the use of genic male sterility is limited because of the high labour cost for maintaining male‐sterile lines. Previous studies using T‐DNA insertional mutagenesis demonstrated that disrupting the expression of oxophytodienoic acid reductase 3 (OPR3), which is involved in the jasmonate biosynthesis pathway, results in a kind of male sterility that can be restored to fertility by exogenous jasmonate in Arabidopsis. Here, we created male‐sterile mutations by editing the second and fourth exons of OsOPR7 in rice through clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated system 9. The induced mutagenesis at these exons resulted in 31.8% and 23.9% male‐sterile plants in the T0 generation, respectively. We screened male‐sterile lines that can be restored to fertility by exogenous methyl jasmonate in the T0, T1 and T2 rice populations and characterized the anther and agronomic traits of the transgenic plants. Results showed the successful generation of male‐sterile lines through the silencing of OsOPR7, the orthologous gene of Arabidopsis OPR3, in a field crop, paving the way for the establishment of a two‐line system for rice hybrid production. The system consists of a male‐sterile line that can be maintained by spraying methyl jasmonate and a restoring line that confers pollen.
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杂种大米可行的男性不育系统：遗传学，生物化学，分子生物学和利用率。
DOI：
10.1186/s12284-014-0013-6
发表时间：
2014-12
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Huang JZ;E ZG;Zhang HL;Shu QY
通讯作者：
Shu QY
影响因子：
16.6
作者：
Cai, Qiang;Yuan, Zheng;Zhang, Dabing
通讯作者：
Zhang, Dabing
影响因子：
5.5
作者：
Basnet, Rasbin;Hussain, Nazim;Shu, Qingyao
通讯作者：
Shu, Qingyao
影响因子：
11.6
作者：
Sanders, PM;Lee, PY;Goldberg, RB
通讯作者：
Goldberg, RB
cDNA 扩增片段长度多态性分析揭示了外源 MeJA 和 GA3 在油菜 (Brassica napus L.) 花中诱导的差异基因表达
影响因子：
4.8
作者：
Pak, Haksong;Li Yu-ling;Jiang Li-xi
通讯作者：
Jiang Li-xi