Increased tartrate-resistant acid phosphatase (TRAP) expression in malignant breast, ovarian and melanoma tissue: an investigational study.
Increased tartrate-resistant acid phosphatase (TRAP) expression in malignant breast, ovarian and melanoma tissue: an investigational study.
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DOI：
10.1186/1471-2407-6-199
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发表时间：
2006-07-25
期刊：
影响因子：
3.8
通讯作者：
Kämmerer U
中科院分区：
文献类型：
--
作者：
Honig A;Rieger L;Kapp M;Krockenberger M;Eck M;Dietl J;Kämmerer U
抗酒石酸酸性磷酸酶（TRAP）是一种金属蛋白酶，属于酸性磷酸酶，已知由破骨细胞表达。它已经被研究作为癌症患者骨转移的标志物。在这项研究中，研究了血清TRAP浓度作为乳腺癌患者骨疾病标志物的价值，我们观察到即使在没有骨转移的患者中也有高浓度的TRAP。为了阐明这一现象，我们研究了TRAP在乳腺癌细胞和其他几种恶性肿瘤细胞中的表达。用ELISA法测定肿瘤患者血清中TRAP浓度。用免疫组化方法分析TRAP在乳腺癌、卵巢癌、宫颈癌和恶性黑色素瘤中的表达。RT-PCR和免疫细胞学检测培养的肿瘤细胞中TRAP的表达。在乳腺癌和卵巢癌患者中观察到血清TRAP浓度显著增加，无论是否存在骨病。TRAP在乳腺癌、卵巢癌和恶性黑色素瘤中表达，而宫颈癌仅显示TRAP的最小表达。TRAP的表达在良性组织中是不存在的，或者比相应的恶性组织中明显得多。TRAP表达也在培养的原代癌细胞和市售细胞系中得到证实。在各种不同肿瘤的细胞中检测到TRAP的过表达。TRAP可作为恶性疾病进展的标志物。它也可能成为未来癌症治疗的潜在靶点。
Tartrate-resistant acid phosphatase (TRAP) is a metalloprotein enzyme that belongs to the acid phosphatases and is known to be expressed by osteoclasts. It has already been investigated as a marker of bone metastases in cancer patients. In this study, which examined the value of serum TRAP concentrations as a marker of bone disease in breast cancer patients, we observed high concentrations of TRAP even in patients without bone metastases. To elucidate this phenomenon, we examined the expression of TRAP in breast cancer cells and the cells of several other malignancies. TRAP concentrations in the serum of tumor patients were determined by ELISA. The expression of TRAP in breast, ovarian, and cervical cancer and malignant melanoma was analyzed by immunohistochemistry. RT-PCR and immunocytology were used to evaluate TRAP expression in cultured tumor cells. A marked increase in serum TRAP concentrations was observed in patients with breast and ovarian cancer, regardless of the presence or absence of bone disease. TRAP expression was found in breast and ovarian cancers and malignant melanoma, while cervical cancer showed only minimal expression of TRAP. Expression of TRAP was absent in benign tissue or was much less marked than in the corresponding malignant tissue. TRAP expression was also demonstrated in cultured primary cancer cells and in commercially available cell lines. Overexpression of TRAP was detected in the cells of various different tumors. TRAP might be useful as a marker of progression of malignant disease. It could also be a potential target for future cancer therapies.
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影响因子：
1.2
作者：
Chen, CJ;Chao, TY;Cheng, SN
通讯作者：
Cheng, SN
影响因子：
3.2
作者：
Nakano, Y;Toyosawa, S;Takano, Y
通讯作者：
Takano, Y
影响因子：
3.2
作者：
Hayman, AR;Bune, AJ;Cox, TM
通讯作者：
Cox, TM
影响因子：
5
作者：
Cabera-Salazar, MA;O'Rourke, E;Barranger, JA
通讯作者：
Barranger, JA
DOI：
10.1007/bf02331443
发表时间：
1996-03-01
期刊：
HISTOCHEMICAL JOURNAL
影响因子：
--
作者：
Janckila, AJ;Yaziji, H;Yam, LT
通讯作者：
Yam, LT