Evaluation of long-term vitamin E insufficiency or excess on bone mass, density, and microarchitecture in rodents.
Evaluation of long-term vitamin E insufficiency or excess on bone mass, density, and microarchitecture in rodents.
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DOI：
10.1016/j.freeradbiomed.2013.09.004
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发表时间：
2013-12
影响因子：
7.4
通讯作者：
Traber, Maret G.
中科院分区：
文献类型：
--
作者：
Iwaniec, Urszula T.;Turner, Russell T.;Smith, Brenda J.;Stoecker, Barbara J.;Rust, Allison;Zhang, Bo;Vasu, Vihas T.;Gohil, Kishorchandra;Cross, Carroll E.;Traber, Maret G.
据报道，高饮食α-生育酚水平会导致生长大鼠骨质减少，而α-生育酚转移蛋白敲除（α-TTP KO）小鼠α-生育酚缺乏会导致松质骨量增加。由于骨质疏松是一种主要与衰老相关的疾病，我们假设α-TTP KO小鼠中与年龄相关的骨质流失会减弱。2岁α-TTP KO和野生型（WT）雄性和雌性小鼠喂养DL-α-生育酚醋酸酯后，采用双能x线吸收仪和显微计算机断层扫描评估其松质和皮质骨量和微结构。与我们的预期相反，无论性别，WT和α-TTP KO小鼠之间的松质骨没有差异，α-TTP KO雄性的皮质骨量低于WT雄性（p < 0.05）。因此，我们评估了骨骼成熟（8.5月龄）雄性Sprague-Dawley大鼠的骨量、密度和股骨近端微结构，这些大鼠分别饲喂低（15 IU/kg）、足（75 IU/kg）或高（500 IU/kg） DL-α-生育酚乙酸饲料13周。低α-生育酚饮食不增加骨量。此外，高α-生育酚饮食没有发现松质或皮质骨量的减少。α-生育酚对老年α-TTP KO小鼠骨量的增加或饲喂高或低水平α-生育酚对骨骼成熟大鼠骨量、密度或微结构的影响均不支持α-生育酚对小鼠骨量、密度或微结构有负面影响的假设。
High dietary α-tocopherol levels reportedly result in osteopenia in growing rats, while α-tocopherol deficiency in α-tocopherol transfer protein knockout (α-TTP KO) mice results in increased cancellous bone mass. Since osteoporosis is a disease associated primarily with aging, we hypothesized that age-related bone loss would be attenuated in α-TTP KO mice. Cancellous and cortical bone mass and microarchitecture were assessed using dual energy x-ray absorptiometry and micro-computed tomography in 2-year-old α-TTP KO and wildtype (WT) male and female mice fed DL-α-tocopherol acetate. In contrast to our expectations, differences in cancellous bone were not detected between WT and α-TTP KO mice in either gender and α-TTP KO males had lower (p < 0.05) cortical bone mass than WT males. We therefore evaluated bone mass, density and microarchitecture in proximal femur of skeletally mature (8.5-months-old) male Sprague-Dawley rats fed diets containing low (15 IU/kg), adequate (75 IU/kg), or high (500 IU/kg diet) DL-α-tocopherol acetate for 13 weeks. Low dietary α-tocopherol did not increase bone mass. Furthermore, no reductions in cancellous or cortical bone mass were detected with high dietary α-tocopherol. Failure to detect increased bone mass in aged α-TTP KO mice or bone changes in skeletally mature rats fed either low or high levels of α-tocopherol does not support the hypothesis that α-tocopherol has a negative impact on bone mass, density or microarchitecture in rodents.
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影响因子：
6
作者：
McNally, AK;Anderson, JM
通讯作者：
Anderson, JM
影响因子：
4.8
作者：
Rickard, David J.;Iwaniec, Urszula T.;Turner, Russell T.
通讯作者：
Turner, Russell T.
影响因子：
5.8
作者：
Ruiz-Ramos, M.;Alberto Vargas, L.;Mendoza-Nunez, V. M.
通讯作者：
Mendoza-Nunez, V. M.
影响因子：
4.1
作者：
Iwaniec, U. T.;Dube, M. G.;Boghossian, S.;Song, H.;Helferich, W. G.;Turner, R. T.;Kalra, S. H.
通讯作者：
Kalra, S. H.
DOI：
10.1155/2012/161527
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Muhammad N;Luke DA;Shuid AN;Mohamed N;Soelaiman IN
通讯作者：
Soelaiman IN