Investigation the interaction between protamine sulfate and CdTe quantum dots with spectroscopic techniques

Investigation the interaction between protamine sulfate and CdTe quantum dots with spectroscopic techniques
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用光谱技术研究硫酸鱼精蛋白和 CdTe 量子点之间的相互作用

DOI:
10.1039/c5ra16586e
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发表时间:
2016-01
期刊:
影响因子:
3.9
通讯作者:
Fangfang Xue,Lingzhi Liu,Yueyao Mi,Heyou Han,Jiangong Liang*
Fangfang Xue,Lingzhi Liu,Yueyao Mi,Heyou Han,Jiangong Liang*
中科院分区:
化学3区
文献类型:
--
作者:
Fangfang Xue,Lingzhi Liu,Yueyao Mi,Heyou Han,Jiangong Liang*

文献摘要

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利用光谱技术研究了硫酸鱼精蛋白与CdTe量子点的相互作用。发现硫酸鱼精蛋白对量子点的荧光有强烈的猝灭作用。根据寿命测量、温度依赖性、紫外-可见吸收光谱、量热滴定和zeta电位测量的结果,提出了一个动态和静态结合的猝灭模型。讨论了结合力的大小,发现该过程与静电相互作用有关。研究结果表明,蛋白质的电荷对量子点的发光有很大的影响。此外,它将有助于量子点与蛋白质相互作用的研究,并促进其在生物系统中的应用。
The interaction between protamine sulfate and CdTe quantum dots (QDs) was investigated in detail by spectroscopic techniques. It was found that the fluorescence of QDs was quenched intensely by protamine sulfate. According to the results of lifetime measurements, temperature dependence, UV-Vis absorption spectra, calorimetric titration and zeta potential measurements, a combined dynamic and static quenching model was proposed. The binding force was also discussed and the process was found to be associated with electrostatic interaction. The results of this work indicated that the charge of protein has great effect on the emission of QDs. Moreover, it should contribute to the studies of interactions of QDs with proteins and promotes its application in biological systems.
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