Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.
Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.
复制标题
DOI：
10.3389/fcimb.2022.1019071
复制
发表时间：
2022
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
--
作者：
SARS-CoV-2疫情发生后，核酸检测迅速走进人们的生活。核酸检测中除了常用的聚合酶链反应（PCR）外，等温扩增法也是重要的核酸检测方法。在置换扩增、滚环扩增等几种常见的等温扩增方法中，重组酶聚合酶扩增（RPA）是近年来受到广泛关注的一种扩增方法。该方法具有操作简单、扩增速度快、反应温度在37-42°C等优点。因此，该方法非常适合于现场检测。然而，爱国军仍有一些缺点。本文主要对RPA的原理、优缺点进行了综述。介绍了RPA在细菌检测、真菌检测、病毒检测、寄生虫检测、耐药基因检测、转基因食品检测、SARS-CoV-2检测等方面的具体应用。希望能将RPA的最新研究进展更好地传递给对RPA感兴趣的读者。
After the outbreak of SARS-CoV-2, nucleic acid testing quickly entered people’s lives. In addition to the polymerase chain reaction (PCR) which was commonly used in nucleic acid testing, isothermal amplification methods were also important nucleic acid testing methods. Among several common isothermal amplification methods like displaced amplification, rolling circle amplification, and so on, recombinase polymerase amplification (RPA) was recently paid more attention to. It had the advantages like a simple operation, fast amplification speed, and reaction at 37-42°C, et al. So it was very suitable for field detection. However, there were still some disadvantages to RPA. Herein, our review mainly summarized the principle, advantages, and disadvantages of RPA. The specific applications of RPA in bacterial detection, fungi detection, virus detection, parasite detection, drug resistance gene detection, genetically modified food detection, and SARS-CoV-2 detection were also described. It was hoped that the latest research progress on RPA could be better delivered to the readers who were interested in RPA.
登录
查看更多内容
影响因子：
4.6
作者：
Lei, Rong;Wang, Xinyi;Jiang, Ning
通讯作者：
Jiang, Ning
影响因子：
5.6
作者：
Ivanov AV;Safenkova IV;Zherdev AV;Dzantiev BB
通讯作者：
Dzantiev BB
影响因子：
4.3
作者：
Barreda-García S;Miranda-Castro R;de-Los-Santos-Álvarez N;Miranda-Ordieres AJ;Lobo-Castañón MJ
通讯作者：
Lobo-Castañón MJ
DOI：
10.1016/j.diagmicrobio.2021.115594
发表时间：
2022-02-01
影响因子：
2.9
作者：
Jiang, Xiaowu;Zhu, Lexin;Zhan, Dongbo
通讯作者：
Zhan, Dongbo
重组酶聚合酶扩增结合聚合物絮凝沉降快速检测食品样品中的金黄色葡萄球菌
DOI：
10.1016/j.ijfoodmicro.2020.108691
发表时间：
2020-10-16
影响因子：
5.4
作者：
Hu, Jinqiang;Wang, Yi;Dong, Caiwen
通讯作者：
Dong, Caiwen