Aptamer-functionalized nanoscale metal-organic frameworks for targeted photodynamic therapy.
Aptamer-functionalized nanoscale metal-organic frameworks for targeted photodynamic therapy.
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用于靶向光动力治疗的适体功能化纳米级金属有机框架
DOI：
10.7150/thno.26768
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发表时间：
2018
期刊：
影响因子：
12.4
通讯作者：
Zhang XB
中科院分区：
文献类型：
--
作者：
Meng HM;Hu XX;Kong GZ;Yang C;Fu T;Li ZH;Zhang XB
光动力学疗法（PDT）已应用于临床肿瘤治疗。在这里，我们报告了一个适体功能化的纳米级金属有机框架靶向PDT。我们的纳米系统可以很容易地制备，并成功地用于靶向PDT，在体外和体内具有显着增强的治疗效果。研究方法：通过结合磷酸封端的适配体与Zr基纳米金属有机骨架（Zr-NMOFs）之间的强结合能力以及光敏剂TMPyP 4嵌入G-四链体DNA结构中，制备了TMPyP 4-G4-适配体-NMOFs。在制备后检查TMPyP 4-G4-适体-NMOFs的特性和光动力学性能。然后，我们研究了它们的稳定性、特异性识别能力和体外光毒性。对于体内实验，将纳米系统瘤内注射到HeLa皮下异种移植肿瘤小鼠模型中。在第0天照射后，在第5天对小鼠进一步注射纳米系统，并再次进行激光照射30分钟。治疗后每2天通过卡尺测量所有小鼠的肿瘤体积和体重。结果：纳米系统诱导靶细胞90%的细胞死亡。相比之下，对照细胞在相同浓度的纳米系统下保持约40%的细胞活力。对于体内实验，纳米系统处理组在整个实验期间保持超过76%的抑制。结论：我们已经证明我们的智能TMPyP 4-G4-sgc 8-NMOFs纳米系统可以用于高效的靶向癌症治疗。
Photodynamic therapy (PDT) has been applied in clinical cancer treatment. Here we report an aptamer-functionalized nanoscale metal-organic framework for targeted PDT. Our nanosystem can be easily prepared and successfully used for targeted PDT with a significantly enhanced therapeutic efficacy in vitro and in vivo. Methods: By combining the strong binding ability between phosphate-terminated aptamers and Zr-based nanoscale metal-organic frameworks (Zr-NMOFs) and the intercalation of photosensitizer TMPyP4 within the G-quadruplex DNA structure, TMPyP4-G4-aptamer-NMOFs were prepared. The characteristics and photodynamic performance of TMPyP4-G4-aptamer-NMOFs were examined after preparation. Then, we studied their stability, specific recognition ability, and phototoxicity in vitro. For in vivo experiments, the nanosystem was intratumorally injected into a HeLa subcutaneous xenograft tumor mouse model. After irradiation on day 0, mice were further injected with the nanosystem on day 5 and were again subjected to laser irradiation for 30 min. Tumor volumes and body weights of all mice were measured by caliper every 2 days after the treatment. Results: The nanosystem induced 90% cell death of targeted cells. In contrast, the control cells maintained about 40% cell viability at the same concentration of nanosystem. For the in vivo experiments, the nanosystem-treated group maintained more than 76% inhibition within the entire experimental period. Conclusion: We have demonstrated that our smart TMPyP4-G4-sgc8-NMOFs nanosystem can be used for targeted cancer therapy with high efficiency.
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影响因子：
17.1
作者：
Shieh, Yen-An;Yang, Shu-Jyuan;Shieh, Ming-Jium
通讯作者：
Shieh, Ming-Jium
影响因子：
15
作者：
Cavka, Jasmina Hafizovic;Jakobsen, Soren;Lillerud, Karl Petter
通讯作者：
Lillerud, Karl Petter
用于癌细胞凋亡和半胱天冬酶响应治疗诊断的卟啉光敏金属有机框架
影响因子：
4.9
作者：
Zhang, Lei;Lei, Jianping;Ju, Huangxian
通讯作者：
Ju, Huangxian
影响因子：
12.4
作者：
Kobayashi H;Watanabe R;Choyke PL
通讯作者：
Choyke PL
影响因子：
15
作者：
Park, Jihye;Jiang, Qin;Zhou, Hong-Cai
通讯作者：
Zhou, Hong-Cai