Oxygen-driven divergence of marine group II archaea reflected by transitions of superoxide dismutases.
Oxygen-driven divergence of marine group II archaea reflected by transitions of superoxide dismutases.
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DOI：
10.1128/spectrum.02033-23
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发表时间：
2024-01-11
影响因子：
3.7
通讯作者：
Xie, Wei
中科院分区：
文献类型：
--
作者：
Qu, Liping;Li, Meng;Gong, Fahui;He, Lei;Li, Minchun;Zhang, Chuanlun;Yin, Kedong;Xie, Wei
超氧阴离子自由基（O2·−），即主要的活性氧（ROS），对细胞功能有害。海洋第二组（MGII）古菌主要生活在富含ROS的海洋环境中，然而，它们对ROS损伤的防御机制仍然未知。本研究综合分析了南海北方MGII古菌16 S rRNA基因扩增产物和宏基因组组合基因组，以及250个已发表的MGII古菌基因组，以探讨其抗氧化机制。结果表明，含铁锰超氧化物歧化酶（Fe/MnSOD）基因组中氧促进的MGII操作分类单元的比例显著高于含镍SOD（NiSOD）基因组，表明含Fe/MnSOD的MGII成员可能比含NiSOD的成员具有上级抗氧化能力。MGII基因组的分子钟分析显示，频繁的物种分化相关的Fe/MnSOD或NiSOD基因。MGII基因组中这些基因的获得/丢失与过去24亿年来大气氧和海洋金属元素浓度的历史变化同步，这表明MGII古菌响应氧水平变化的抗氧化能力的变化可能会随着时间的推移而驱动它们的分歧。活性氧（Reactive oxygen species，ROS）是一系列引起生物体氧化应激的物质，包括超氧阴离子。海洋古菌群II（MGII）主要生活在表层海水中，暴露于大量的活性氧中。因此，了解MGII的抗氧化能力非常重要。研究发现，Fe/MnSOD可能更适合MGII抵抗氧化损伤，氧浓度和SOD金属辅因子的变化在MGII 17个分支对SOD的选择中起重要作用，进而影响MGII的物种分化。总的来说，这项研究提供了深入了解这些未开垦的海洋古菌与地球系统的共同进化历史。
The superoxide anion radical (O2•−), the primary reactive oxygen species (ROS), is detrimental to cellular functions. Marine group II (MGII) archaea predominantly inhabit surface ocean environments that are rich in ROS; however, their defense mechanisms against ROS damage remain unknown. In this study, we integrated the analyses of archaeal 16S rRNA gene amplicons and metagenome-assemblage genomes of MGII archaea from the northern South China Sea and 250 other previously published MGII genomes to investigate their antioxidant mechanisms. The results show that the proportion of oxygen-promoted MGII operational taxonomic units in the iron/manganese superoxide dismutase (Fe/MnSOD) gene-containing group was significantly higher than that in the nickel SOD (NiSOD) group, suggesting that MGII members with Fe/MnSOD may have superior antioxidant capacity relative to members possessing NiSOD. Molecular clock analysis of MGII genomes revealed frequent species divergence correlating to the differentiation of Fe/MnSOD or NiSOD genes. The acquisition/loss of these genes in the MGII genomes was synchronized with the historical changes in atmospheric oxygen and oceanic metal element concentrations over the last 2.4 billion years, suggesting that variations in the antioxidant capacity of MGII archaea in response to changing oxygen levels may have driven their divergences over time. Reactive oxygen species (ROS), including superoxide anion, is a series of substances that cause oxidative stress for all organisms. Marine group II (MGII) archaea are mainly live in the surface seawater and exposed to considerable ROS. Therefore, it is important to understand the antioxidant capacity of MGII. Our research found that Fe/Mn- superoxide dismutase (Fe/MnSOD) may be more suitable for MGII to resist oxidative damage, and the changes in oxygen concentrations and SOD metallic cofactors play an important role in the selection of SOD by the 17 clades of MGII, which in turn affects the species differentiation of MGII. Overall, this study provides insight into the co-evolutionary history of these uncultivated marine archaea with the earth system.
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影响因子：
64.8
作者：
Frigaard, NU;Martinez, A;DeLong, EF
通讯作者：
DeLong, EF
影响因子：
5
作者：
Fasnacht M;Polacek N
通讯作者：
Polacek N
DOI：
10.1007/978-3-642-13179-0_4
发表时间：
2011-01-01
期刊：
OXYGEN AND THE EVOLUTION OF LIFE
影响因子：
--
作者：
Decker, Heinz;van Holde, Kensal E.
通讯作者：
van Holde, Kensal E.
影响因子：
10.7
作者：
Hoang DT;Chernomor O;von Haeseler A;Minh BQ;Vinh LS
通讯作者：
Vinh LS
DOI：
10.1093/bioinformatics/btp348
发表时间：
2009-08-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Capella-Gutiérrez S;Silla-Martínez JM;Gabaldón T
通讯作者：
Gabaldón T