Melatonin enhances DNA repair capacity possibly by affecting genes involved in DNA damage responsive pathways.
Melatonin enhances DNA repair capacity possibly by affecting genes involved in DNA damage responsive pathways.
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DOI：
10.1186/1471-2121-14-1
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发表时间：
2013-01-07
期刊：
影响因子：
--
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Liu R;Fu A;Hoffman AE;Zheng T;Zhu Y
褪黑激素是一种参与昼夜节律调节的激素样物质，已被证明可以保护细胞免受氧化 DNA 损伤并抑制肿瘤发生。在当前的研究中，我们使用碱性 DNA 彗星测定法研究了褪黑激素对乳腺癌 (MCF-7) 和结肠癌 (HCT-15) 细胞系中 DNA 链断裂的影响。我们的结果表明，在暴露于诱变剂（甲磺酸甲酯，MMS）后，与未经褪黑素处理的细胞相比，经褪黑素预处理的细胞具有显着更短的 Olive 尾矩，表明褪黑素处理后 DNA 修复能力增强。我们进一步检查了褪黑素预处理的 MCF-7 细胞在暴露于致癌物后的全基因组基因表达，并检测到涉及多种 DNA 损伤反应途径的许多基因的表达改变。使用基于网络和通路的生物信息学分析进一步分析表达改变的基因的功能相关性。顶级功能网络被定义为与“DNA 复制、重组和修复、基因表达和癌症”相关。这些发现表明，褪黑激素可能通过影响涉及 DNA 损伤反应途径的几个关键基因来增强 DNA 修复能力。
Melatonin, a hormone-like substance involved in the regulation of the circadian rhythm, has been demonstrated to protect cells against oxidative DNA damage and to inhibit tumorigenesis. In the current study, we investigated the effect of melatonin on DNA strand breaks using the alkaline DNA comet assay in breast cancer (MCF-7) and colon cancer (HCT-15) cell lines. Our results demonstrated that cells pretreated with melatonin had significantly shorter Olive tail moments compared to non-melatonin treated cells upon mutagen (methyl methanesulfonate, MMS) exposure, indicating an increased DNA repair capacity after melatonin treatment. We further examined the genome-wide gene expression in melatonin pretreated MCF-7 cells upon carcinogen exposure and detected altered expression of many genes involved in multiple DNA damage responsive pathways. Genes exhibiting altered expression were further analyzed for functional interrelatedness using network- and pathway-based bioinformatics analysis. The top functional network was defined as having relevance for “DNA Replication, Recombination, and Repair, Gene Expression, [and] Cancer”. These findings suggest that melatonin may enhance DNA repair capacity by affecting several key genes involved in DNA damage responsive pathways.
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