A Novel ex vivo Mouse Mesometrium Culture Model for Investigating Angiogenesis in Microvascular Networks.
A Novel ex vivo Mouse Mesometrium Culture Model for Investigating Angiogenesis in Microvascular Networks.
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DOI：
10.1159/000489102
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发表时间：
2018
影响因子：
1.7
通讯作者：
Murfee WL
中科院分区：
文献类型：
--
作者：
Suarez-Martinez AD;Bierschenk S;Huang K;Kaplan D;Bayer CL;Meadows SM;Sperandio M;Murfee WL
发展模型，纳入完整的微血管网络，使多细胞动力学血管生成过程中的调查。本实验室引进了大鼠肠系膜培养模型作为实验工具，并将用小鼠组织进行强化。由于小鼠系膜是无血管的，因此另一种选择是小鼠子宫系膜，子宫角的结缔组织。这项研究的目的是证明小鼠子宫系膜含有微血管网络，可以培养来研究血管生成过程中的多细胞动力学。将从C57 B1/6雌性小鼠收获的子宫系膜组织在含血清的培养基中培养长达7天。PECAM、NG 2、αSMA和LYVE-1标记分别鉴定了内皮细胞、周细胞、平滑肌细胞和淋巴管内皮细胞。这些细胞包括微血管网络与微动脉，微静脉和毛细血管。与第0天相比，培养3天和5天时每个血管长度的毛细血管芽增加（第0天：0.08±0.01，第3天：3.19±0.78，第5天：2.49±0.05芽/mm; p<0.05）。来自FlkEGFP小鼠的培养组织的延时成像展示了该模型用于谱系研究的用途。这种影响得到了内皮细胞从一个芽跳到另一个芽的鉴定的支持。这些结果介绍了一种新的培养模型，用于研究在真实离体微血管网络中血管生成过程中的多细胞动力学。
Development of models that incorporate intact microvascular networks enables the investigation of multicellular dynamics during angiogenesis. Our laboratory introduced the rat mesentery culture model as such tool, which would be enhanced with mouse tissue. Since mouse mesentery is avascular, an alternative is mouse mesometrium, connective tissue of uterine horns. The study’s objective was to demonstrate that mouse mesometrium contains microvascular networks that can be cultured to investigate multicellular dynamics during angiogenesis. Harvested mesometrium tissues from C57Bl/6 female mice were cultured in media with serum for up to 7 days. PECAM, NG2, αSMA, and LYVE-1 labeling identified endothelial cells, pericytes, smooth muscle cells, and lymphatic endothelial cells, respectively. These cells comprised microvascular networks with arterioles, venules and capillaries. Compared to day 0, capillary sprouts per vascular length was increased by 3 and 5 days in culture (Day 0: 0.08±0.01, Day 3: 3.19±0.78, Day 5: 2.49±0.05 sprouts/mm; p<0.05). Time-lapse imaging of cultured tissues from FlkEGFP mice showcases use of the model for lineage studies. The impact is supported by the identification of endothelial cell jumping from one sprout to another. These results introduce a novel culture model for investigating multicellular dynamics during angiogenesis in real-ex vivo microvascular networks.
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影响因子：
1.7
作者：
Stapor PC;Sweat RS;Dashti DC;Betancourt AM;Murfee WL
通讯作者：
Murfee WL
DOI：
10.1111/micc.12107
发表时间：
2014-05
期刊：
Microcirculation (New York, N.Y. : 1994)
影响因子：
--
作者：
Kelly-Goss MR;Sweat RS;Stapor PC;Peirce SM;Murfee WL
通讯作者：
Murfee WL
DOI：
10.1152/ajpheart.00552.2012
发表时间：
2013-01-01
影响因子：
4.8
作者：
Stapor, Peter C.;Azimi, Mohammad S.;Murfee, Walter L.
通讯作者：
Murfee, Walter L.
影响因子：
1.4
作者：
Sweat, Richard S.;Stapor, Peter C.;Murfee, Walter L.
通讯作者：
Murfee, Walter L.
影响因子：
7.8
作者：
Nehls, V;Drenckhahn, D
通讯作者：
Drenckhahn, D