Fungal genotype determines survival of Drosophila melanogaster when competing with Aspergillus nidulans.
Fungal genotype determines survival of Drosophila melanogaster when competing with Aspergillus nidulans.
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DOI：
10.1371/journal.pone.0190543
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Kempken F
中科院分区：
文献类型：
--
作者：
Regulin A;Kempken F
真菌产生的次级代谢产物种类惊人，其中一些属于生物界中毒性最大的化合物。一些真菌代谢产物具有抗杀虫特性，这可能使真菌在与昆虫竞争环境资源时具有优势。使用果蝇/构巢曲霉生态模型系统来评估次级代谢物突变基因型，我们发现veA等位基因在昆虫/真菌对抗中的重要作用超过了其他因素如LaeA的影响。VeA沿着LaeA是一个转录复合体的成员，控制着A. nidulans。然而，历史上的突变veA等位基因，veA 1减少次级代谢产物的输出，已被用于该模式生物的许多研究。为了测试该等位基因在我们的系统中的显著性，在对抗试验中评估构巢曲霉veA野生型、veA 1、ΔveA和ΔlaeA，以分析产卵活性和幼虫存活率。veA 1基因背景导致幼虫存活率显著增加。成蝇几乎只在veA 1，ΔveA或ΔlaeA遗传背景下观察到，表明天鹅绒复合物在昆虫/真菌相互作用中的作用。使用veA 1突变体时，这种效果最为显著。因此，幼虫在对抗中的生存受到真菌基因型的高度影响。
Fungi produce an astonishing variety of secondary metabolites, some of which belong to the most toxic compounds in the living world. Several fungal metabolites have anti-insecticidal properties which may yield advantages to the fungus in competition with insects for exploitation of environmental resources. Using the Drosophila melanogaster/Aspergillus nidulans ecological model system to assess secondary metabolite mutant genotypes, we find a major role for the veA allele in insect/fungal confrontations that exceeds the influence of other factors such as LaeA. VeA along with LaeA is a member of a transcriptional complex governing secondary metabolism in A. nidulans. However, historically a mutant veA allele, veA1 reduced in secondary metabolite output, has been used in many studies of this model organism. To test the significance of this allele in our system, Aspergillus nidulans veA wild type, veA1, ΔveA and ΔlaeA were evaluated in confrontation assays to analyze egg laying activity, and the survival rate of larvae. The veA1 genetic background led to a significant increase of larval survival. Adult flies were observed almost exclusively on veA1, ΔveA or ΔlaeA genetic backgrounds, suggesting a role for the velvet complex in insect/fungal interactions. This effect was most profound using the veA1 mutant. Hence, larval survival in confrontations is highly affected by the fungal genotype.
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影响因子：
3.7
作者：
Alkahyyat F;Ni M;Kim SC;Yu JH
通讯作者：
Yu JH
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4.4
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Chiang, Yi-Ming;Szewczyk, Edyta;Oakley, Berl R.
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Oakley, Berl R.
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Butchko, Robert A. E.;Brown, Daren W.;Wiemann, Philipp
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通讯作者：
Keller, Nancy P.
影响因子：
3.6
作者：
Bok, Jin Woo;Noordermeer, Daan;Keller, Nancy P.
通讯作者：
Keller, Nancy P.