Facile preparation of photodegradable hydrogels by photopolymerization.
Facile preparation of photodegradable hydrogels by photopolymerization.
复制标题
DOI：
10.1016/j.polymer.2013.02.018
复制
发表时间：
2013-04-03
期刊：
影响因子：
4.6
通讯作者：
Lin, Chien-Chi
中科院分区：
文献类型：
--
作者：
Ki, Chang Seok;Shih, Han;Lin, Chien-Chi
可光降解的水凝胶已经成为研究和指导细胞行为以及递送药物的强大材料平台。该技术的前提是使用细胞相容性光源来切割水凝胶内的接头，从而以时空受控的方式引起基质刚度的降低或基质束缚的生物分子的释放。最常用的光降解单元是含有硝基苄基部分的分子，其吸收紫外线（UV）至较低可见波长（约280至450 nm）的光。由于迄今为止文献中报道的光降解连接体和水凝胶都对UV光敏感，因此高效的UV介导的光聚合不太可能用作制备这些水凝胶的方法。因此，目前可用的可光降解的水凝胶通过氧化还原介导的自由基聚合、乳液聚合、迈克尔型加成反应或正交点击化学形成。在这里，我们报告的第一个光降解聚（乙二醇）为基础的水凝胶系统制备的逐步增长的光聚合。通过常规固相肽合成法合成的模型光不稳定肽交联剂含有用于逐步生长硫醇-烯光点击反应的末端半胱氨酸和用于光裂解的肽骨架中的UV敏感的2-硝基苯丙氨酸残基。通过调节紫外光暴露时间和强度实现该肽的光解。通过高效可见光介导的硫醇-烯光点击反应，使用非切割型光引发剂曙红-Y，使含有光不稳定肽交联剂的光可降解水凝胶的光聚合成为可能。通过原位光流变仪证实了快速凝胶化。在控制的波长和强度下的泛光UV照射用于证明这些光聚合水凝胶的光降解性。
Photodegradable hydrogels have emerged as a powerful material platform for studying and directing cell behaviors, as well as for delivering drugs. The premise of this technique is to use a cytocompatible light source to cleave linkers within a hydrogel, thus causing reduction of matrix stiffness or liberation of matrix-tethered biomolecules in a spatial-temporally controlled manner. The most commonly used photodegradable units are molecules containing nitrobenzyl moieties that absorb light in the ultraviolet (UV) to lower visible wavelengths (~280 to 450 nm). Because photodegradable linkers and hydrogels reported in the literature thus far are all sensitive to UV light, highly efficient UV-mediated photopolymerizations are less likely to be used as the method to prepare these hydrogels. As a result, currently available photodegradable hydrogels are formed by redox-mediated radical polymerizations, emulsion polymerizations, Michael-type addition reactions, or orthogonal click chemistries. Here, we report the first photodegradable poly(ethylene glycol)-based hydrogel system prepared by step-growth photopolymerization. The model photolabile peptide cross-linkers, synthesized by conventional solid phase peptide synthesis, contained terminal cysteines for step-growth thiol-ene photo-click reactions and a UV-sensitive 2-nitrophenylalanine residue in the peptide backbone for photo-cleavage. Photolysis of this peptide was achieved through adjusting UV light exposure time and intensity. Photopolymerization of photodegradable hydrogels containing photolabile peptide cross-linkers was made possible via a highly efficient visible light-mediated thiol-ene photo-click reaction using a non-cleavage type photoinitiator eosin-Y. Rapid gelation was confirmed by in situ photo-rheometry. Flood UV irradiation at controlled wavelength and intensity was used to demonstrate the photodegradability of these photopolymerized hydrogels.
登录
查看更多内容
影响因子：
3.4
作者：
Klinger, Daniel;Landfester, Katharina
通讯作者：
Landfester, Katharina
影响因子：
14
作者：
Kizilel, S;Sawardecker, E;Pérez-Luna, VH
通讯作者：
Pérez-Luna, VH
影响因子：
5.5
作者：
Fairbanks BD;Singh SP;Bowman CN;Anseth KS
通讯作者：
Anseth KS
影响因子：
29.4
作者：
Fairbanks, Benjamin D;Schwartz, Michael P;Halevi, Alexandra E;Nuttelman, Charles R;Bowman, Christopher N;Anseth, Kristi S
通讯作者：
Anseth, Kristi S
影响因子：
14
作者：
Kloxin, April M.;Benton, Julie A.;Anseth, Kristi S.
通讯作者：
Anseth, Kristi S.