A Novel Regulation of K-antigen Capsule Synthesis in Porphyromonas gingivalis Is Driven by the Response Regulator PG0720-Directed Antisense RNA.
A Novel Regulation of K-antigen Capsule Synthesis in Porphyromonas gingivalis Is Driven by the Response Regulator PG0720-Directed Antisense RNA.
复制标题
DOI：
10.3389/froh.2021.701659
复制
发表时间：
2021
影响因子：
--
通讯作者：
Davey ME
中科院分区：
文献类型：
--
作者：
Kim HM;Ranjit DK;Walker AR;Getachew H;Progulske-Fox A;Davey ME
牙周病原菌牙龈卟啉单胞菌菌株 W83 表现出至少三种不同的表面聚糖，特别是两种类型的脂多糖（O-LPS 和 A-LPS）和 K 抗原胶囊。尽管 K 抗原荚膜对牙龈卟啉单胞菌的毒力很重要，但人们对于如何调节参与这种表面聚糖合成的基因表达却知之甚少。 K 抗原荚膜合成所需的基因位于编码许多转录物的基因座中，包括操纵子（PG0104 至 PG0121，生成约 19.4-kb 转录物），其在 5' 端附近包含非编码 77-bp 反向重复序列 (77 bpIR) 区域。此前，我们鉴定了一个 550 核苷酸的反义 RNA 分子（称为 SuGR，即反义表面聚糖调节剂），其编码在影响表面聚糖合成的 77 bpIR 元件中。在本研究中，我们证明DNA结合反应调节剂PG0720可以结合asSuGR的启动子区域并激活asSuGR的表达，表明PG0720可能间接影响有义链表达的K抗原荚膜操纵子的转录水平。数据显示，与亲本菌株相比，PG0720基因的缺失导致表面多糖的呈现存在缺陷，定量RT-PCR（qPCR）分析确定，PG0720突变体中参与K抗原荚膜合成的基因的总体表达下调。此外，PG0720缺失突变体的缺陷通过互补得到恢复。重要的是，PG0720 缺失突变体表现出毒力降低。总之，我们的数据表明，反应调节剂 PG0720 调节 asSuGR 的表达，asSuGR 是一种反式作用反义 RNA 分子，参与调节牙龈卟啉单胞菌菌株 W83 中表面多糖的产生。这些数据进一步证明表面聚糖是关键的毒力决定因素，并显着增进了我们对控制牙龈卟啉单胞菌 K 抗原胶囊（关键毒力决定因素）合成的分子机制的理解。
The periodontal pathogen Porphyromonas gingivalis strain W83 displays at least three different surface glycans, specifically two types of lipopolysaccharides (O-LPS and A-LPS) and K-antigen capsule. Despite the importance of K-antigen capsule to the virulence of P. gingivalis, little is known as to how expression of genes involved in the synthesis of this surface glycan is regulated. The genes required for K-antigen capsule synthesis are located in a locus that encodes a number of transcripts, including an operon (PG0104 to PG0121, generating ~19.4-kb transcript) which contains a non-coding 77-bp inverted repeat (77 bpIR) region near the 5'-end. Previously, we identified a 550-nucleotide antisense RNA molecule (designated asSuGR for antisense Surface Glycan Regulator) encoded within the 77-bpIR element that influences the synthesis of surface glycans. In this study, we demonstrate that the DNA-binding response regulator PG0720 can bind the promoter region of asSuGR and activate expression of asSuGR, indicating that PG0720 may indirectly influence transcript levels of the K-antigen capsule operon expressed from the sense strand. The data show that deletion of the PG0720 gene confers a defect in the presentation of surface polysaccharides compared with the parent strain and quantitative RT-PCR (qPCR) analysis determined that the overall expression of genes involved in K-antigen capsule synthesis were down-regulated in the PG0720 mutant. Furthermore, the defects of the PG0720 deletion mutant were restored by complementation. Importantly, the PG0720 deletion mutant showed reduced virulence. Altogether, our data show that the response regulator PG0720 regulates expression of asSuGR, a trans-acting antisense RNA molecule involved in modulating the production of surface polysaccharides in P. gingivalis strain W83. The data provide further evidence that surface glycans are key virulence determinants and significantly advances our understanding of the molecular mechanisms controlling the synthesis of P. gingivalis K-antigen capsule, a key virulence determinant.
登录
查看更多内容
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
4.4
作者：
Gómez-Lozano M;Marvig RL;Tulstrup MV;Molin S
通讯作者：
Molin S
影响因子：
--
作者：
Laine, ML;van Winkelhoff, AJ
通讯作者：
van Winkelhoff, AJ
影响因子：
3.7
作者：
Dou, Y.;Aruni, W.;Fletcher, H. M.
通讯作者：
Fletcher, H. M.
影响因子：
18.6
作者：
Lewis JP
通讯作者：
Lewis JP