Activation of cortical 5-HT(3) receptor-expressing interneurons induces NO mediated vasodilatations and NPY mediated vasoconstrictions.
Activation of cortical 5-HT(3) receptor-expressing interneurons induces NO mediated vasodilatations and NPY mediated vasoconstrictions.
复制标题
表达受体表达的皮质5-HT的激活不会诱导介导的血管扩张和NPY介导的血管收缩。
DOI：
10.3389/fncir.2012.00050
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发表时间：
2012
影响因子：
3.5
通讯作者：
Rancillac A
中科院分区：
文献类型：
--
作者：
Perrenoud Q;Rossier J;Férézou I;Geoffroy H;Gallopin T;Vitalis T;Rancillac A
GABA能中间神经元是皮质活动的局部整合者，已报道其通过产生血管活性分子的能力和其对邻近血管的丰富神经支配参与脑血流量（CBF）的控制。它们形成高度多样化的群体，其中除了对多巴胺能上行通路的反应性之外，表达5-羟色胺3A受体（5-HT 3A）的中间神经元共享共同的发育起源。我们最近发现，这些神经元重组两个不同的亚群内的躯体感觉皮层：神经肽Y（NPY）的表达interneurons，显示类似的神经胶质细胞和血管活性肠肽（VIP）的表达双极/双簇的interneurons的形态学特性。本研究的目的是通过监测血管直径的变化，确定这些神经元群体在控制血管张力的作用，使用红外视频显微镜在小鼠新皮层切片。浴应用1-（3-氯苯基）双胍盐酸盐（mCPBG），5-HT 3R激动剂，诱导收缩（30%）和扩张（70%）的穿通小动脉颗粒上层。所有的血管收缩在NPY受体拮抗剂（BIBP 3226）的存在下被取消，这表明它们是由NPY释放引起的。血管舒张持续存在的VIP受体拮抗剂VPAC 1（PG-97-269），而他们的神经元一氧化氮（NO）合酶（nNOS）抑制剂，L-NNA的存在下被阻断。总之，这些结果强烈地表明，通过胆碱能输入激活新皮层5-HT 3A表达的中间神经元可以诱导NO介导的血管舒张和NPY介导的血管收缩。
GABAergic interneurons are local integrators of cortical activity that have been reported to be involved in the control of cerebral blood flow (CBF) through their ability to produce vasoactive molecules and their rich innervation of neighboring blood vessels. They form a highly diverse population among which the serotonin 5-hydroxytryptamine 3A receptor (5-HT3A)-expressing interneurons share a common developmental origin, in addition to the responsiveness to serotonergic ascending pathway. We have recently shown that these neurons regroup two distinct subpopulations within the somatosensory cortex: Neuropeptide Y (NPY)-expressing interneurons, displaying morphological properties similar to those of neurogliaform cells and Vasoactive Intestinal Peptide (VIP)-expressing bipolar/bitufted interneurons. The aim of the present study was to determine the role of these neuronal populations in the control of vascular tone by monitoring blood vessels diameter changes, using infrared videomicroscopy in mouse neocortical slices. Bath applications of 1-(3-Chlorophenyl)biguanide hydrochloride (mCPBG), a 5-HT3R agonist, induced both constrictions (30%) and dilations (70%) of penetrating arterioles within supragranular layers. All vasoconstrictions were abolished in the presence of the NPY receptor antagonist (BIBP 3226), suggesting that they were elicited by NPY release. Vasodilations persisted in the presence of the VIP receptor antagonist VPAC1 (PG-97-269), whereas they were blocked in the presence of the neuronal Nitric Oxide (NO) Synthase (nNOS) inhibitor, L-NNA. Altogether, these results strongly suggest that activation of neocortical 5-HT3A-expressing interneurons by serotoninergic input could induces NO mediated vasodilatations and NPY mediated vasoconstrictions.
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影响因子：
3
作者：
Gholipour, Taha;Ghasemi, Mehdi;Dehpour, Ahmad Reza
通讯作者：
Dehpour, Ahmad Reza
影响因子：
2.5
作者：
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影响因子：
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作者：
CHEDOTAL, A;UMBRIACO, D;HAMEL, E
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HAMEL, E
影响因子：
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BREDT, DS;GLATT, CE;SNYDER, SH
通讯作者：
SNYDER, SH
影响因子：
34.7
作者：
Ascoli, Giorgio A.;Alonso-Nanclares, Lidia;Anderson, Stewart A.;Barrionuevo, German;Benavides-Piccione, Ruth;Burkhalter, Andreas;Buzsaki, Gyoergy;Cauli, Bruno;DeFelipe, Javier;Fairen, Alfonso;Feldmeyer, Dirk;Fishell, Gord;Fregnac, Yves;Freund, Tamas F.;Gardner, Daniel;Gardner, Esther P.;Goldberg, Jesse H.;Helmstaedter, Moritz;Hestrin, Shaul;Karube, Fuyuki;Kisvarday, Zoltan F.;Lambolez, Bertrand;Lewis, David A.;Marin, Oscar;Markram, Henry;Munoz, Alberto;Packer, Adam;Petersen, Carl C. H.;Rockland, Kathleen S.;Rossier, Jean;Rudy, Bernardo;Somogyi, Peter;Staiger, Jochen F.;Tamas, Gabor;Thomson, Alex M.;Toledo-Rodriguez, Maria;Wang, Yun;West, David C.;Yuste, Rafael
通讯作者：
Yuste, Rafael