Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs.
Sperm function, mitochondrial activity and in vivo fertility are associated to their mitochondrial DNA content in pigs.
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DOI：
10.1186/s40104-023-00988-0
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发表时间：
2024-02-01
影响因子：
7
通讯作者：
Yeste, Marc
中科院分区：
文献类型：
--
作者：
Llavanera, Marc;Mateo-Otero, Yentel;Vinolas-Verges, Estel;Bonet, Sergi;Yeste, Marc
尽管精子中线粒体丰度较低，但线粒体在这种细胞类型中具有多种功能，包括能量产生、信号传导和钙调节。据报道，在人类中，精子线粒体 DNA 含量 (mtDNAc) 与精子功能和生育能力呈负相关。然而，mtDNAc 与家畜精子功能之间的关联仍有待探索。因此，本研究旨在阐明 mtDNAc 与猪精子功能和受精潜力之间的联系。针对猪精子优化了 mtDNAc 定量的 qPCR 方法，随后探讨了该参数与精子活力、运动学、线粒体活性和生育力的关联。 首先，qPCR 方法对猪精子中的 mtDNAc 定量敏感且高效。通过使用这种技术，观察到 mtDNAc 与精子活力、线粒体活性和体内（但体外）生育结果相关。具体来说，mtDNAc 较低的精子表现出更大的活力，但线粒体活性和细胞内活性氧含量降低。有趣的是，与 mtDNAc 较高的样本相比，mtDNAc 较低的样本表现出较高的受孕率和分娩率，但体外受精率和胚胎发育相似。 这些发现丰富了我们对 mtDNAc 与精子生物学关系的理解，并为未来研究利用该参数作为牲畜精子功能和生育力的分子预测因子奠定了基础。 在线版本包含可在 10.1186/s40104-023-00988-0 获取的补充材料。
Despite their low abundance in sperm, mitochondria have diverse functions in this cell type, including energy production, signalling and calcium regulation. In humans, sperm mitochondrial DNA content (mtDNAc) has been reported to be negatively linked to sperm function and fertility. Yet, the association between mtDNAc and sperm function in livestock remains unexplored. For this reason, this study aimed to shed some light on the link between mtDNAc and sperm function and fertilising potential in pigs. A qPCR method for mtDNAc quantification was optimised for pig sperm, and the association of this parameter with sperm motility, kinematics, mitochondrial activity, and fertility was subsequently interrogated. First, the qPCR method was found to be sensitive and efficient for mtDNAc quantification in pig sperm. By using this technique, mtDNAc was observed to be associated to sperm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. Specifically, sperm with low mtDNAc were seen to exhibit greater motility but decreased mitochondrial activity and intracellular reactive oxygen species. Interestingly, samples with lower mtDNAc showed higher conception and farrowing rates, but similar in vitro fertilisation rates and embryo development, when compared to those with greater mtDNAc. These findings enrich our comprehension of the association of mtDNAc with sperm biology, and lay the foundation for future research into employing this parameter as a molecular predictor for sperm function and fertility in livestock. The online version contains supplementary material available at 10.1186/s40104-023-00988-0.
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影响因子：
6
作者：
Chen PR;Redel BK;Kerns KC;Spate LD;Prather RS
通讯作者：
Prather RS
影响因子：
11.4
作者：
Nolfi-Donegan D;Braganza A;Shiva S
通讯作者：
Shiva S
影响因子：
6.1
作者：
Amaral, Alexandra;Ramalho-Santos, Joao;St John, Justin C.
通讯作者：
St John, Justin C.
影响因子：
6.7
作者：
Kao, SH;Chao, HT;Wei, YH
通讯作者：
Wei, YH
DOI：
10.1007/978-1-4939-1875-1_3
发表时间：
2015
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Rooney, John P;Ryde, Ian T;Sanders, Laurie H;Howlett, Evan H;Colton, Meryl D;Germ, Kaylyn E;Mayer, Greg D;Greenamyre, J Timothy;Meyer, Joel N
通讯作者：
Meyer, Joel N