Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage.
Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage.
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DOI：
10.1016/s0022-2836(03)00865-9
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发表时间：
2003-08-29
影响因子：
5.6
通讯作者：
Gorbalenya AE
中科院分区：
文献类型：
--
作者：
Snijder EJ;Bredenbeek PJ;Dobbe JC;Thiel V;Ziebuhr J;Poon LL;Guan Y;Rozanov M;Spaan WJ;Gorbalenya AE
利用新近发表的SARS冠状病毒（SARS-CoV）基因组序列预测了新发现的SARS冠状病毒的基因组结构和表达策略。鉴定了14个推定的开放阅读框，其中12个被预测从8个亚基因组mRNA的嵌套组表达。实验证实了这些mRNA在SARS-CoV感染的细胞中的合成。4382和7073个氨基酸残基的SARS-CoV复制酶多聚蛋白被预测被两种病毒蛋白酶切割成16个亚基（使SARS-CoV蛋白的总数达到28）。复制酶基因的系统发育分析，使用一个遥远的相关torovirus作为外群，表明，尽管有一些独特的功能，SARS冠状病毒是最密切相关的第2组冠状病毒。在第2组冠状病毒中发现了细胞RNA加工酶的远缘同源物，其中4个在SARS-CoV中是保守的。这些新发现的病毒酶将冠状病毒RNA合成机制置于一个全新的视角。此外，与先前描述的病毒酶一起，它们将成为设计旨在控制SARS-CoV进一步传播的抗病毒策略的重要靶标。
The genome organization and expression strategy of the newly identified severe acute respiratory syndrome coronavirus (SARS-CoV) were predicted using recently published genome sequences. Fourteen putative open reading frames were identified, 12 of which were predicted to be expressed from a nested set of eight subgenomic mRNAs. The synthesis of these mRNAs in SARS-CoV-infected cells was confirmed experimentally. The 4382- and 7073 amino acid residue SARS-CoV replicase polyproteins are predicted to be cleaved into 16 subunits by two viral proteinases (bringing the total number of SARS-CoV proteins to 28). A phylogenetic analysis of the replicase gene, using a distantly related torovirus as an outgroup, demonstrated that, despite a number of unique features, SARS-CoV is most closely related to group 2 coronaviruses. Distant homologs of cellular RNA processing enzymes were identified in group 2 coronaviruses, with four of them being conserved in SARS-CoV. These newly recognized viral enzymes place the mechanism of coronavirus RNA synthesis in a completely new perspective. Furthermore, together with previously described viral enzymes, they will be important targets for the design of antiviral strategies aimed at controlling the further spread of SARS-CoV.
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影响因子：
3.5
作者：
Gorbalenya AE;Koonin EV;Lai MM
通讯作者：
Lai MM
影响因子：
3.7
作者：
Den Boon JA;Snijder EJ;Locker JK;Horzinek MC;Rottier PJ
通讯作者：
Rottier PJ
影响因子：
5.4
作者：
BAKER, SC;YOKOMORI, K;LAI, MMC
通讯作者：
LAI, MMC
影响因子：
3.8
作者：
Chouljenko, VN;Lin, XQ;Gorbalenya, AE
通讯作者：
Gorbalenya, AE
影响因子：
56.9
作者：
Anand, K;Ziebuhr, J;Hilgenfeld, R
通讯作者：
Hilgenfeld, R