Linking the region-specific tissue microstructure to the biaxial mechanical properties of the porcine left anterior descending artery.
Linking the region-specific tissue microstructure to the biaxial mechanical properties of the porcine left anterior descending artery.
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DOI：
10.1016/j.actbio.2022.07.036
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发表时间：
2022-09-15
影响因子：
9.7
通讯作者：
Lee, Chung-Hao
中科院分区：
文献类型：
--
作者：
Pineda-Castillo, Sergio A.;Aparicio-Ruiz, Santiago;Burns, Madison M.;Laurence, Devin W.;Bradshaw, Elizabeth;Gu, Tingting;Holzapfel, Gerhard A.;Lee, Chung-Hao
关键词：
冠状动脉粥样硬化是世界范围内导致死亡的主要原因。提高对冠状动脉微结构力学的理解是开发治疗工具和外科手术的基础。虽然冠状动脉的被动双轴特性已经被广泛研究，但它们的区域差异以及组织微结构与力学之间的关系还没有得到充分的表征。在这项研究中，我们研究了猪左前降支(LADA)近端、内侧和远端的被动双轴机械特性和微结构特性。我们还尝试将LADA的双轴应力-拉伸响应与其各自对偏振光的双折射响应联系起来，以获得与载荷相关的微结构变化的信息。我们发现，LADA的延伸性在近端到远端方向降低，内侧区域比其他两个区域表现出更多的非均匀力学行为。我们还观察到了高度动态的微观结构行为，其中纤维族根据负载重新排列。此外，我们还发现，远端区域的微观结构显示出沿动脉纵轴高度排列的纤维。为了验证这种微结构特征，我们用多光子显微镜对LADA标本进行成像，观察到外膜微结构从近端的随机纤维网络过渡到远端的高度排列的纤维。我们的发现可能为理解冠状动脉力学提供新的视角，并有助于组织工程血管移植物的发展，目前由于其与天然组织在力学特性和微观结构特征方面的不匹配而受到限制。
Coronary atherosclerosis is the main cause of death worldwide. Advancing the understanding of coronary microstructure-based mechanics is fundamental for the development of therapeutic tools and surgical procedures. Although the passive biaxial properties of the coronary arteries have been extensively explored, their regional differences and the relationship between tissue microstructure and mechanics have not been fully characterized. In this study, we characterized the passive biaxial mechanical properties and microstructural properties of the proximal, medial, and distal regions of the porcine left anterior descending artery (LADA). We also attempted to relate the biaxial stress-stretch response of the LADA and its respective birefringent responses to the polarized light for obtaining information about the load-dependent microstructural variations. We found that the LADA extensibility is reduced in the proximal-to-distal direction and that the medial region exhibits more heterogeneous mechanical behavior than the other two regions. We have also observed highly dynamic microstructural behavior where fiber families realign themselves depending on loading. In addition, we found that the microstructure of the distal region exhibited highly aligned fibers along the longitudinal axis of the artery. To verify this microstructural feature, we imaged the LADA specimens with multi-photon microscopy and observed that the adventitia microstructure transitioned from a random fiber network in the proximal region to highly aligned fibers in the distal region. Our findings may offer new perspectives for understanding coronary mechanics and aid in the development of tissue-engineered vascular grafts, which are currently limited due to their mismatch with native tissue in terms of mechanical properties and microstructural features.
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DOI：
10.1038/s43856-021-00063-7
发表时间：
2022
期刊：
COMMUNICATIONS MEDICINE
影响因子：
--
作者：
Blum, Kevin M;Zbinden, Jacob C;Ramachandra, Abhay B;Lindsey, Stephanie E;Szafron, Jason M;Reinhardt, James W;Heitkemper, Megan;Best, Cameron A;Mirhaidari, Gabriel J M;Chang, Yu-Chun;Ulziibayar, Anudari;Kelly, John;Shah, Kejal V;Drews, Joseph D;Zakko, Jason;Miyamoto, Shinka;Matsuzaki, Yuichi;Iwaki, Ryuma;Ahmad, Hira;Daulton, Robbie;Musgrave, Drew;Wiet, Matthew G;Heuer, Eric;Lawson, Emily;Schwarz, Erica;McDermott, Michael R;Krishnamurthy, Rajesh;Krishnamurthy, Ramkumar;Hor, Kan;Armstrong, Aimee K;Boe, Brian A;Berman, Darren P;Trask, Aaron J;Humphrey, Jay D;Marsden, Alison L;Shinoka, Toshiharu;Breuer, Christopher K
通讯作者：
Breuer, Christopher K
影响因子：
2.4
作者：
Chen H;Kassab GS
通讯作者：
Kassab GS
DOI：
10.3758/bf03203630
发表时间：
1996-02-01
期刊：
BEHAVIOR RESEARCH METHODS INSTRUMENTS & COMPUTERS
影响因子：
--
作者：
Erdfelder, E;Faul, F;Buchner, A
通讯作者：
Buchner, A
影响因子：
2.4
作者：
Cheng, Shaokoon;Clarke, Elizabeth C.;Bilston, Lynne E.
通讯作者：
Bilston, Lynne E.
影响因子：
2.4
作者：
Humphrey, J. D.;Eberth, J. F.;Dye, W. W.;Gleason, R. L.
通讯作者：
Gleason, R. L.