Role of non-coding RNAs in maintaining primary airway smooth muscle cells.
Role of non-coding RNAs in maintaining primary airway smooth muscle cells.
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DOI：
10.1186/1465-9921-15-58
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发表时间：
2014-05-16
影响因子：
5.8
通讯作者：
Chung KF
中科院分区：
文献类型：
--
作者：
Perry MM;Tsitsiou E;Austin PJ;Lindsay MA;Gibeon DS;Adcock IM;Chung KF
呼吸道平滑肌(ASM)细胞维持其自身的增殖率，并参与呼吸道的炎症反应，这种作用可被用于治疗呼吸道疾病的皮质类固醇所抑制。我们测定了皮质类固醇、地塞米松和胎牛血清(FCS)处理后原代ASM细胞中mRNAs、microRNAs(MiRNAs)和长非编码RNA物种(LncRNAs)的差异表达。用基因芯片和实时荧光定量聚合酶链式反应检测细胞内mR NA、miRNA和lncRNA的表达。少数miRNA(包括miR-150、−371-5p、−718、−940、−1181、−1207-5p、−1915和−3663-3p)在地塞米松和FCS作用下减少。这些miRNAs的信使核糖核酸靶基因表达增加。MRNA表达的变化与ASM肌动蛋白细胞骨架的调控有关。我们还观察到在地塞米松和FCS作用下，lncRNAs的表达变化，包括自然反义、假基因、内含子和基因间的lncRNAs。我们证实了其中三个基因LINC00882、LINC00883、PVT1及其转录激活因子c-myc的表达发生了变化。我们认为其中4个lincRNA(RP11-46A10.4、LINC00883、BCYRN1和LINC00882)作为4个−(miR-150、−371-5P、−940、miRNA1207-5P)的miRNA“海绵”。这种原代ASM细胞表型的体外模型与几个ncRNAs的调控有关。它们的鉴定允许进行体外功能实验，以建立与主要ASM表型以及哮喘和慢性阻塞性肺疾病(COPD)等呼吸道疾病的因果关系。
The airway smooth muscle (ASM) cell maintains its own proliferative rate and contributes to the inflammatory response in the airways, effects that are inhibited by corticosteroids, used in the treatment of airways diseases. We determined the differential expression of mRNAs, microRNAs (miRNAs) and long noncoding RNA species (lncRNAs) in primary ASM cells following treatment with a corticosteroid, dexamethasone, and fetal calf serum (FCS). mRNA, miRNA and lncRNA expression was measured by microarray and quantitative real-time PCR. A small number of miRNAs (including miR-150, −371-5p, −718, −940, −1181, −1207-5p, −1915, and −3663-3p) were decreased following exposure to dexamethasone and FCS. The mRNA targets of these miRNAs were increased in expression. The changes in mRNA expression were associated with regulation of ASM actin cytoskeleton. We also observed changes in expression of lncRNAs, including natural antisense, pseudogenes, intronic lncRNAs, and intergenic lncRNAs following dexamethasone and FCS. We confirmed the change in expression of three of these, LINC00882, LINC00883, PVT1, and its transcriptional activator, c-MYC. We propose that four of these lincRNAs (RP11-46A10.4, LINC00883, BCYRN1, and LINC00882) act as miRNA ‘sponges’ for 4 miRNAs (miR-150, −371-5p, −940, −1207-5p). This in-vitro model of primary ASM cell phenotype was associated with the regulation of several ncRNAs. Their identification allows for in-vitro functional experimentation to establish causality with the primary ASM phenotype, and in airway diseases such as asthma and chronic obstructive pulmonary disease (COPD).
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影响因子：
4.1
作者：
Ma L;Bajic VB;Zhang Z
通讯作者：
Zhang Z
ERCC1，BAG-1，BRCA1，RRM1和TUBB3的mRNA表达的组合分析，以预测接受辅助化疗的非小细胞肺癌患者的预后。
DOI：
10.1186/1756-9966-31-25
发表时间：
2012-03-23
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
Leng XF;Chen MW;Xian L;Dai L;Ma GY;Li MH
通讯作者：
Li MH
影响因子：
3.9
作者：
Chung, Kian Fan
通讯作者：
Chung, Kian Fan
影响因子：
9.7
作者：
Liu, Hanshao;Zhu, Lin;Xiao, Huasheng
通讯作者：
Xiao, Huasheng
影响因子：
14.9
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.