Comparative genomics of transport proteins in developmental bacteria: Myxococcus xanthus and Streptomyces coelicolor.
Comparative genomics of transport proteins in developmental bacteria: Myxococcus xanthus and Streptomyces coelicolor.
复制标题
DOI：
10.1186/1471-2180-13-279
复制
发表时间：
2013-12-05
期刊：
影响因子：
4.2
通讯作者：
Saier MH Jr
中科院分区：
文献类型：
--
作者：
Getsin I;Nalbandian GH;Yee DC;Vastermark A;Paparoditis PC;Reddy VS;Saier MH Jr
两个最大的全测序原核生物基因组是放线菌，coelicolor链霉菌（Sco）和δ-变形菌，黄粘球菌（Mxa），两者都是分化，产孢，产生抗生素的土壤微生物。虽然Sco和Mxa的基因组大小相同（约9 Mbp），但Sco的基因比Mxa的平均小10%。令人惊讶的是，Sco的可识别转运蛋白比Mxa多93%。这是因为Sco扩增了几种特定类型的转运蛋白基因，而Mxa扩增的程度要小得多。扩增是底物和家族特异性的。例如，Sco而不是Mxa放大了其电压门控离子通道，但没有放大其水通道蛋白和机械敏感通道。Sco（而非Mxa）也扩增了主要促进剂超家族（MFS） DHA2家族的药物外排泵（49比6）、APC家族的氨基酸转运蛋白（17比2）、abc型糖转运蛋白（85比6）和几个家族的有机阴离子转运蛋白。Sco没有扩增大多数其他类型的转运蛋白。相对于Sco， Mxa选择性地扩增了一个大鳄出口者家族（16对1），这与Mxa比Sco产生更多大鳄的观察结果一致。除了电子传递载体外，这两种生物中发现的转运体类型之间的相关性很差，这表明它们对分化和代谢需求的解决方案是独立进化的。在这两种重要的模式生物及其近亲中，提出了许多意想不到和令人惊讶的观察结果，并对可识别的转运蛋白的生理功能以及尚未发现的转运系统的存在进行了预测。结果提供了洞察进化过程，其中两个不同的原核生物进化的复杂性，特别是通过选择性染色体基因扩增。
Two of the largest fully sequenced prokaryotic genomes are those of the actinobacterium, Streptomyces coelicolor (Sco), and the δ-proteobacterium, Myxococcus xanthus (Mxa), both differentiating, sporulating, antibiotic producing, soil microbes. Although the genomes of Sco and Mxa are the same size (~9 Mbp), Sco has 10% more genes that are on average 10% smaller than those in Mxa. Surprisingly, Sco has 93% more identifiable transport proteins than Mxa. This is because Sco has amplified several specific types of its transport protein genes, while Mxa has done so to a much lesser extent. Amplification is substrate- and family-specific. For example, Sco but not Mxa has amplified its voltage-gated ion channels but not its aquaporins and mechano-sensitive channels. Sco but not Mxa has also amplified drug efflux pumps of the DHA2 Family of the Major Facilitator Superfamily (MFS) (49 versus 6), amino acid transporters of the APC Family (17 versus 2), ABC-type sugar transport proteins (85 versus 6), and organic anion transporters of several families. Sco has not amplified most other types of transporters. Mxa has selectively amplified one family of macrolid exporters relative to Sco (16 versus 1), consistent with the observation that Mxa makes more macrolids than does Sco. Except for electron transport carriers, there is a poor correlation between the types of transporters found in these two organisms, suggesting that their solutions to differentiative and metabolic needs evolved independently. A number of unexpected and surprising observations are presented, and predictions are made regarding the physiological functions of recognizable transporters as well as the existence of yet to be discovered transport systems in these two important model organisms and their relatives. The results provide insight into the evolutionary processes by which two dissimilar prokaryotes evolved complexity, particularly through selective chromosomal gene amplification.
登录
查看更多内容
影响因子：
11.3
作者：
Berleman JE;Kirby JR
通讯作者：
Kirby JR
DOI：
10.1126/science.1227215
发表时间：
2012-09-28
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chng SS;Xue M;Garner RA;Kadokura H;Boyd D;Beckwith J;Kahne D
通讯作者：
Kahne D
影响因子：
3.4
作者：
Procópio, Rudi Emerson de Lima;Silva, Ingrid Reis da;Araújo, Janete Magali de
通讯作者：
Araújo, Janete Magali de
影响因子：
2.8
作者：
Colson, Severine;van Wezel, Gilles P.;Rigali, Sebastien
通讯作者：
Rigali, Sebastien
影响因子：
2.9
作者：
Chatterjee, Srirupa;Chaudhury, Sukanya;McShan, Andrew C.;Kaur, Kawaljit;De Guzman, Roberto N.
通讯作者：
De Guzman, Roberto N.