High expression of RNF169 is associated with poor prognosis in pancreatic adenocarcinoma by regulating tumour immune infiltration.
High expression of RNF169 is associated with poor prognosis in pancreatic adenocarcinoma by regulating tumour immune infiltration.
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DOI：
10.3389/fgene.2022.1022626
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发表时间：
2022
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
背景：胰腺腺癌（PAAD）是一种高度致命、侵袭性强的肿瘤，预后不良。然而，RNF169在PAAD中的预后价值及其相关机制尚未阐明。在本研究中，我们旨在探索 PAAD 中的预后相关基因，特别是 RNF169，并确定 PAAD 的新型潜在预后预测因子。 方法：利用GEPIA和UALCAN数据库研究RNF169在PAAD中的表达及其预后价值。使用TIMER和TISIDB确定RNF169表达与免疫浸润之间的相关性。与starBase进行相关性分析，以确定lncRNA-miRNA-RNF169的潜在调控轴。 结果:数据显示PAAD组织中RNF169 mRNA的表达水平高于正常组织。 RNF169 高表达与 PAAD 预后不良相关。此外，TISIDB和TIMER数据库分析显示，PAAD中RNF169的表达与肿瘤免疫浸润呈正相关。相关分析表明，长链非编码RNA（lncRNA）AL049555.1和微小RNA（miRNA）hsa-miR-324-5p参与RNF169的表达，构成控制PAAD进展的潜在调控轴。基因本体论 (GO) 和京都基因与基因组百科全书 (KEGG) 富集分析表明，RNF169 通过 TNF、Hippo、JAK-STAT 和 Toll 样受体信号通路等途径在 PAAD 中发挥作用。 结论：综上所述，ncRNA介导的RNF169表达上调可能影响微环境中的免疫细胞浸润；因此，它可以用作 PAAD 的预后生物标志物和潜在的治疗靶点。
Background: Pancreatic adenocarcinoma (PAAD) is a highly deadly and aggressive tumour with a poor prognosis. However, the prognostic value of RNF169 and its related mechanisms in PAAD have not been elucidated. In this study, we aimed to explore prognosis-related genes, especially RNF169 in PAAD and to identify novel potential prognostic predictors of PAAD. Methods: The GEPIA and UALCAN databases were used to investigate the expression and prognostic value of RNF169 in PAAD. The correlation between RNF169 expression and immune infiltration was determined by using TIMER and TISIDB. Correlation analysis with starBase was performed to identify a potential regulatory axis of lncRNA-miRNA-RNF169. Results: The data showed that the level of RNF169 mRNA expression in PAAD tissues was higher than that in normal tissues. High RNF169 expression was correlated with poor prognosis in PAAD. In addition, analysis with the TISIDB and TIMER databases revealed that RNF169 expression was positively correlated with tumour immune infiltration in PAAD. Correlation analysis suggested that the long non-coding RNA (lncRNA) AL049555.1 and the microRNA (miRNA) hsa-miR-324-5p were involved in the expression of RNF169, composing a potential regulatory axis to control the progression of PAAD. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses indicated that RNF169 plays a role in PAAD through pathways such as TNF, Hippo, JAK-STAT and Toll-like receptor signaling. Conclusion: In summary, the upregulation of RNF169 expression mediated by ncRNAs might influence immune cell infiltration in the microenvironment; thus, it can be used as a prognostic biomarker and a potential therapeutic target in PAAD.
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