Evaluation of extraction methods for pharmacologically active compounds from anticonvulsant traditional Chinese medicines: Gou Teng, Tian Ma, Jiang Can using DART-TOF-MS.
Evaluation of extraction methods for pharmacologically active compounds from anticonvulsant traditional Chinese medicines: Gou Teng, Tian Ma, Jiang Can using DART-TOF-MS.
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DOI：
10.1039/d0ay02015j
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发表时间：
2021-02-21
期刊：
影响因子：
--
通讯作者：
Peace MR
中科院分区：
文献类型：
--
作者：
Karin KN;Poklis JL;Peace MR
中草药（CHMs）被归类为膳食补充剂。与西药的相互作用，污染物或掺假物的存在，或错误标记或误用中药材可能导致在死亡调查中无法发现的毒理学紧急情况。实验室必须能够有效地分析疑似中药的病例。评估了五种提取方法从草药基质中提取药理学活性化合物的能力：水、乙醇、微波辅助（MAE）、乙醇、氯仿和酸洗。采用直接实时质谱法（DART-MS）比较了购自中国北京和美国纽约的勾藤、天麻和姜参粉末和提取物中的抗惊厥和其他药理活性成分。每次提取大约使用0.25 g浸渍草药。水提取和乙醇提取均为简单的液体提取。对于MAE，将草药粉末浸泡在65%的乙醇中，微波加热，浓缩。乙醇：氯仿提取包括1:1乙醇：氯仿浸泡、超声和浓缩。在酸洗提取中，将草药粉末浸泡在乙酸中，然后加入氢氧化钠，己烷提取，乙酸乙酯中重构。采用Jeol JMS T100LC AccuTOF DART-MS进行阳性和阴性分析。在评价的方法中，没有一种提取方法能提取出三种中药的所有活性成分。三种产品中，MAE提取物的药理活性成分最多，而酸洗提取物的药理活性成分最少。从不同来源购买的勾藤确实表现出不同的药理活性成分，可能来自不同的物种。随着天然产品的普及，从天然基质中快速分析具有药理活性的分析物对公共卫生至关重要。微波辅助萃取与DART-MS提供了有效的分析从草药基质分析物。
Chinese herbal medicines (CHMs) are classified as dietary supplements. Interactions with western medications, the presence of contaminants or adulterants, or a mis-labeled or mis-used CHM may lead to toxicological emergencies that can be undetected in death investigations. Laboratories must be able to efficiently analyze cases in which CHMs are suspected. Five extractions were evaluated for their ability to extract pharmacologically active compounds from herbal matrices: water, ethanol, microwave-assisted (MAE), ethanol : chloroform, and acid-wash. Anticonvulsive and other pharmacologically active compounds in Gou Teng, Tian Ma, and Jiang Can purchased from Beijing, China and New York were compared in the powder and the extracts using Direct Analysis in Real Time-Mass Spectrometry (DART-MS). Approximately 0.25 g of macerated herb was used per extraction. The water and ethanol extractions were simple liquid extractions. For the MAE, powdered herb was soaked in 65% ethanol, microwaved, and concentrated. The ethanol : chloroform extraction involved soaking in 1 : 1 ethanol : chloroform, sonication, and concentration. In the acid-wash extraction, powdered herb was soaked in acetic acid, followed by addition of sodium hydroxide, hexane extraction, and reconstitution in ethyl acetate. The powdered herbs and extracts were analyzed using a Jeol JMS T100LC AccuTOF DART-MS in positive and negative mode. Of the evaluated methods, no single extraction worked for all active compounds from the three CHMs. The MAE extract contained the most pharmacologically active compounds, while the acid-wash contained the least for the three products. Gou Teng purchased from different sources did exhibit a difference in pharmacologically active compounds, potentially from different species. With the popularity of natural products, rapid analysis of pharmacologically active analytes from natural matrices is critical for public health. Microwave-assisted extraction with DART-MS provides efficient analysis of analytes from herbal matrices.
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影响因子：
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作者：
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