Effect of Treadmill Exercise and Hydrogen-rich Water Intake on Serum Oxidative and Anti-oxidative Metabolites in Serum of Thoroughbred Horses.
Effect of Treadmill Exercise and Hydrogen-rich Water Intake on Serum Oxidative and Anti-oxidative Metabolites in Serum of Thoroughbred Horses.
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DOI：
10.1294/jes.24.1
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发表时间：
2013
影响因子：
--
通讯作者：
Aida H
中科院分区：
文献类型：
--
作者：
Tsubone H;Hanafusa M;Endo M;Manabe N;Hiraga A;Ohmura H;Aida H
本研究旨在阐明跑步机锻炼的纯种马（n = 5，3至7岁）的氧化应激和抗氧化功能的变化，使用最近开发的测量氧化应激血清d-ROM和抗氧化标记物BAP的技术。此外，还检查了跑步机运动前经鼻饲给予富氢水 (HW) 或安慰剂水对这些参数的影响。每匹马都接受最大水平的跑步机锻炼，其中马匹以 13.2 ± 0.84 m/sec 的平均速度疲惫不堪。采集血液样本 4 次，即在跑步机运动前 30 分钟摄入 HW 或安慰剂水之前、运动前（运动前）、运动后（运动后）和运动后 30 分钟。所有马匹的 d-ROM 和 BAP 值在运动后均显着增加。与运动前安慰剂试验相比，硬件试验中 d-ROM 的增加往往较低。在 HW 和安慰剂治疗试验中，BAP 的增加相当可观，约为运动前值的 150%。在两项治疗试验中，BAP/d-ROMs 比率在运动后显着升高，而在 HW 试验中，运动前也观察到显着升高。除安慰剂试验中的 BAP 和 BAP/d-ROM 外，运动后 30 分钟，BAP、d-ROM 和 BAP/d-ROM 比率趋于下降。这些结果表明，在剧烈运动的马中，氧化应激和抗氧化功能同时显着升高，并表明 HW 可能具有一定的抗氧化功效。
The present study aimed to clarify changes of oxidative stress and antioxidative functions in treadmill-exercised Thoroughbred horses (n=5, 3 to 7 years old), using recently developed techniques for measurement of serum d-ROMs for oxidative stress, and BAP for antioxidative markers. Also, the effect of nasogastric administration of hydrogen-rich water (HW) or placebo water preceding the treadmill exercise on these parameters was examined. Each horse was subjected to a maximum level of treadmill exercise in which the horses were exhausted at an average speed of 13.2 ± 0.84 m/sec. Blood samples were taken 4 times, immediately before the intake of HW or placebo water at 30 min preceding the treadmill exercise, immediately before the exercise (pre-exercise), immediately after the exercise (post-exercise) and at 30 min following the exercise. In all horses, both d-ROMs and BAP values significantly increased at post-exercise. The increase in d-ROMs tended to be lower in the HW trial, as compared to the placebo trial at pre-exercise. The increase in BAP was considerable at approximately 150% of the pre-exercise values in both the HW and placebo treatment trials. The BAP/d-ROMs ratio was significantly elevated at post-exercise in both treatment trials, while a significant elevation was also observed at pre-exercise in the HW trial. BAP, d-ROM, and the BAP/d-ROM ratio tended to decline at 30 min after the exercise, except BAP and BAP/d-ROMs in the placebo trial. These results demonstrate that the marked elevation of oxidative stress and anitioxidative functions occurred simultaneously in the intensively exercised horses, and suggest a possibility that HW has some antioxidative efficacy.
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DOI：
10.1007/bf00376495
发表时间：
1996-08-01
期刊：
EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY AND OCCUPATIONAL PHYSIOLOGY
影响因子：
--
作者：
Mills, PC;Smith, NC;Marlin, DJ
通讯作者：
Marlin, DJ
影响因子：
6.8
作者：
Iamele, L;Fiocchi, R;Vernocchi, A
通讯作者：
Vernocchi, A
影响因子：
4.5
作者：
Komatsu, Fumio;Kagawa, Yasuo;Purvee, Baatar
通讯作者：
Purvee, Baatar
影响因子：
1.6
作者：
Kotani, Kazuhiko;Yamada, Toshiyuki
通讯作者：
Yamada, Toshiyuki
影响因子：
5.5
作者：
McArdle, A;van der Meulen, J;Jackson, MJ
通讯作者：
Jackson, MJ