Effect of endurance training on retinol-binding protein 4 gene expression and its protein level in adipose tissue and the liver in diabetic rats induced by a high-fat diet and streptozotocin.
Effect of endurance training on retinol-binding protein 4 gene expression and its protein level in adipose tissue and the liver in diabetic rats induced by a high-fat diet and streptozotocin.
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DOI：
10.1111/jdi.12186
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发表时间：
2014-09
影响因子：
3.2
通讯作者：
Omidfar K
中科院分区：
文献类型：
--
作者：
Mansouri M;Nikooie R;Keshtkar A;Larijani B;Omidfar K
本研究旨在研究哪些组织中视黄醇结合蛋白4（RBP 4）表达的降低可能有助于耐力训练后血清RBP 4和胰岛素抵抗（IR）的改善。将7周龄雄性Wistar大鼠随机分为4组，包括对照组（C）、训练组（T）、糖尿病对照组（DC）和训练糖尿病组（TD）。在8周龄时，通过高脂肪饮食和腹膜内注射低剂量链脲佐菌素（STZ; 35 mg/kg）诱导糖尿病。T组和TD组大鼠在电动跑步机上进行为期7周的运动计划（15-20 m/min，20 min/天，持续5周），而C组和DC组大鼠在笼中保持久坐不动。分别使用真实的实时聚合酶链反应和蛋白质印迹法评估组织中RBP 4的基因表达和蛋白质水平，同时使用酶联免疫吸附测定试剂盒测量血清RBP 4。运动显著改善TD组的IR和降低血清RBP 4浓度。这种血清RBP 4的降低伴随着内脏脂肪组织中RBP 4蛋白表达的降低。相反，运动对TD组肝脏和皮下脂肪组织中RBP 4的表达没有显著影响。运动还显著降低内脏脂肪组织和肌肉中RBP 4基因的表达，而运动对肝脏RBP 4信使核糖核酸表达的影响不显著。本研究表明，RBP 4降低耐力训练效果的机制可能与降低STZ诱导的糖尿病大鼠脂肪组织中RBP 4信使核糖核酸表达及其蛋白水平有关。
The present study was designed to investigate from which tissues the decrease in retinol‐binding protein 4 (RBP4) expression could contribute to the improvement of serum RBP4 and insulin resistance (IR) after endurance training. Male 7‐week‐old Wistar rats were randomly assigned into four groups including control (C), trained (T), diabetic control (DC) and trained diabetic (TD). At 8 weeks‐of‐age, diabetes was induced by a high‐fat diet and intraperitoneal injection of low‐dose streptozotocin (STZ; 35 mg/kg). Rats in the T and TD groups carried out a 7‐week exercise program on a motorized treadmill (15–20 m/min for 20 min/day for 5 weeks), whereas the C and DC remained sedentary in their cages. Tissues gene expression and protein levels of RBP4 were assessed by using real‐time polymerase chain reaction and western blot, respectively, while serum RBP4 was measured using an enzyme‐linked immunosorbent assay kit. Exercise significantly improved IR and reduced serum concentration of RBP4 in the TD group. This reduction of serum RBP4 was accompanied by decreased RBP4 protein expression in visceral fat tissue. In contrast, exercise had no significant effect on RBP4 expression in liver and subcutaneous fat tissue in the TD group. Exercise also significantly decreased RBP4 gene expression in visceral fat tissue and muscle, whereas the effect of exercise on liver RBP4 messenger ribonucleic acid expression was not significant. The present study showed that the mechanism for RBP4 reducing the effect of endurance training could involve decreased RBP4 messenger ribonucleic acid expression and its protein level in adipose tissue in STZ‐induced diabetic rats.
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影响因子：
5.1
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通讯作者：
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