A lectin gene is involved in the defense of Pleurotus ostreatus against the mite predator Tyrophagus putrescentiae.
A lectin gene is involved in the defense of Pleurotus ostreatus against the mite predator Tyrophagus putrescentiae.
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DOI：
10.3389/fmicb.2023.1191500
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
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腐食酪螨（Tyrophagus putrescentiae）是一种重要的食用菌害虫，广泛分布于世界各地。为控制害虫而过度喷洒化学品与环境污染、健康风险、杀虫剂抗药性发展和食品安全有关。寄主抗药性可以是可持续的和具有成本效益的，并提供有效和经济的害虫防治。以往的研究表明，平菇已经进化出有效的防御机制。腐殖酸攻击，但潜在的机制仍不清楚。在这里，我们报告了一个凝集素基因从糙皮侧耳菌丝体，Polec 2，诱导真菌抗螨放牧。Polec 2属于半乳糖凝集素样凝集素，编码一个具有β-sandwith-fold结构域的蛋白。Polec 2在糙皮侧耳中的过表达导致活性氧（ROS）/丝裂原活化蛋白激酶（MAPK）信号通路、水杨酸（SA）和茉莉酸（JA）生物合成的激活。结果表明，随着温度的升高，过氧化氢酶（CAT）、过氧化物酶（POD）和超氧化物歧化酶（SOD）的抗氧化活性增强，茉莉酸（JA）、茉莉酸-异亮氨酸（JA-Ile）和茉莉酸甲酯（MeJA）的产生增加，T.腐食菌的进食和抑制其人口。我们还提供了一个概述的系统发育分布凝集素在22个真菌基因组。本研究结果有助于揭示平菇防御螨类捕食者的分子机制，为真菌-食真菌相互作用的分子基础研究和抗虫基因的基因挖掘提供理论依据。
The storage mite, Tyrophagus putrescentiae, found worldwide in many habitats, is an important pest of edible mushrooms. Excessive chemical spraying for pest control has been linked to environmental pollution, health risks, insecticide resistance development, and food safety. Host resistance can be sustainable and cost-effective and provide effective and economical pest control. Previous studies have reported that the oyster mushroom Pleurotus ostreatus has evolved effective defense mechanisms against T. putrescentiae attack, but the underlying mechanism remains unclear. Here we report that a lectin gene from P. ostreatus mycelia, Polec2, induced fungal resistance to mite grazing. Polec2 belongs to a galectin-like lectin classification, encoding a protein with β-sandwith-fold domain. Overexpression of Polec2 in P. ostreatus led to activation of the reactive oxygen species (ROS)/mitogen-activated protein kinases (MAPKs) signaling pathway, salicylic acid (SA), and jasmonate (JA) biosynthesis. The activation resulted in bursts of antioxidant activities of catalases (CAT), peroxidases (POD), superoxide dismutases (SOD), and increased production of SA, JA, jasmonic acid-isoleucine (JA-Ile) and jasmonic acid methyl ester (MeJA), accompanied by reduced T. putrescentiae feeding and suppressed its population. We also provide an overview of the phylogenetic distribution of lectins across 22 fungal genomes. Our findings shed light on the molecular mechanisms of P. ostreatus’ defense against the mite predator and will be useful in investigating the molecular basis of fungi-fungivory interactions and gene mining for pest-resistance genes.
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影响因子：
3
作者：
Albores, Silvana;Mora, Paola;Franco Fraguas, Laura
通讯作者：
Franco Fraguas, Laura
DOI：
10.1126/science.1236011
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
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通讯作者：
Staskawicz BJ
影响因子：
14.9
作者：
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通讯作者：
Bork P
影响因子：
3.8
作者：
Kawagishi, H;Mitsunaga, SI;Chiba, S
通讯作者：
Chiba, S
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影响因子：
56.9
作者：
Deng, Yiwen;Zhai, Keran;He, Zuhua
通讯作者：
He, Zuhua