Interfacial biodegradation of phenanthrene in bacteria-carboxymethyl cellulose-stabilized Pickering emulsions
Interfacial biodegradation of phenanthrene in bacteria-carboxymethyl cellulose-stabilized Pickering emulsions
复制标题
细菌-羧甲基纤维素稳定的皮克林乳液中菲的界面生物降解
DOI：
10.1007/s00253-022-11952-9
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发表时间：
2022-05
影响因子：
5
通讯作者：
Jiameng Zhang
中科院分区：
文献类型：
--
作者：
Tao Pan;Congyang Liu;Meini Wang;Jiameng Zhang
多环芳烃在非水相液体（NAPL）中有限的生物利用度限制了其降解。研究了亲水性细菌Moraxella sp.CFP312在壳聚糖和羧甲基纤维素（CMC）两种聚合物辅助下对正十四烷中菲的降解。壳聚糖和羧甲基纤维素均提高了CFP 312细胞的疏水性，并使CFP 312细胞的接触角分别从30.4增加到78.5和88.5。CMC使菲的降解率从45%提高到近100%，而壳聚糖对菲的降解率没有任何提高。结果表明，CMC比壳聚糖更能促进CFP 312稳定Pickering乳液。在细菌-CMC复合体系中，油脂被分散成小液滴，获得高乳化指数和大比表面积。此外，根据细菌-CMC乳液液滴的显微图像，我们观察到液滴表面被CFP 312细胞紧密覆盖。因此，加入CMC的CFP 312细胞可以在油水界面利用油相中的菲。本研究为利用亲水性细菌有效降解NAPLs中的疏水性化合物提供了一种新的策略。要点：·皮克林乳液中菲的生物降解·由亲水性CFP 312与CMC结合稳定的皮克林乳液。·CFP 312在油水界面上降解菲。
The limited bioavailability of PAHs in non-aqueous phase liquid (NAPL) limits their degradation. The biodegradation of phenanthrene in n-tetradecane by hydrophilic bacterium Moraxella sp. CFP312 was studied with the assistance of two polymers, chitosan and carboxymethyl cellulose (CMC). Both chitosan and CMC improved the cell hydrophobicity of CFP312 and increased the contact angle of CFP312 cells from 30.4 to 78.5 and 88.5, respectively. However, CMC increased the degradation ratio of phenanthrene from 45 to nearly 100%, while chitosan did not cause any improvement. We found that CMC was more effective than chitosan in promoting CFP312 to stabilize Pickering emulsion. In the bacteria-CMC complex system, oil was dispersed into small droplets to obtain a high emulsification index and large specific surface area. Moreover, according to the microscopic image of the bacteria-CMC emulsion droplet, we observed that the droplet surface was tightly covered by the CFP312 cells. Therefore, CFP312 cells joined with CMC can utilize phenanthrene in oil phase at the oil-water interface. This study will offer a new strategy for effective microbial degradation of hydrophobic compounds in NAPLs by hydrophilic bacteria. KEY POINTS: • Biodegradation of phenanthrene in Pickering emulsions • Pickering emulsions stabilized by hydrophilic CFP312 joined with CMC. • Phenanthrene was degraded by CFP312 at oil-water interface.
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DOI：
10.1007/978-981-15-6607-3_6
发表时间：
2021
期刊：
--
影响因子：
--
作者：
D. C. Ali;Zhilong Wang
通讯作者：
D. C. Ali;Zhilong Wang
影响因子：
11.4
作者：
B. Mahanty;K. Pakshirajan;V. V. Dasu-V.
通讯作者：
B. Mahanty;K. Pakshirajan;V. V. Dasu-V.
影响因子：
3.4
作者：
Mohanty, Gita;Mukherji, Suparna
通讯作者：
Mukherji, Suparna
影响因子：
1.4
作者：
Hamdan, Leila J.;Fulmer, Preston A.
通讯作者：
Fulmer, Preston A.
DOI：
10.1016/j.colsurfb.2012.08.064
发表时间：
2013-02
期刊：
Colloids and surfaces. B, Biointerfaces
影响因子：
--
作者：
Sagarika Mohanty;S. Mukherji
通讯作者：
Sagarika Mohanty;S. Mukherji