Gene expression down-regulation in CD90+ prostate tumor-associated stromal cells involves potential organ-specific genes.
Gene expression down-regulation in CD90+ prostate tumor-associated stromal cells involves potential organ-specific genes.
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DOI：
10.1186/1471-2407-9-317
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发表时间：
2009-09-08
期刊：
影响因子：
3.8
通讯作者：
Liu AY
中科院分区：
文献类型：
--
作者：
Pascal LE;Goo YA;Vêncio RZ;Page LS;Chambers AA;Liebeskind ES;Takayama TK;True LD;Liu AY
前列腺间质是上皮细胞分化和发育的关键介质，在前列腺癌的发生和发展中可能起着重要作用。肿瘤相关间质以CD90/THY1表达增加为特征。这些间质细胞的分离和鉴定可以为肿瘤微环境的生物学研究提供有价值的信息。采用细胞分选法从肿瘤标本中分离出CD90+间质纤维肌细胞，并用DNA芯片进行分析。数据集分析用于比较组织学正常和肿瘤相关基质细胞的基因表达。为了进行比较，还从膀胱中分离和分析了基质细胞。与肿瘤相关的间质细胞被发现降低了与平滑肌分化有关的基因的表达，这些基因在前列腺中检测到，但在膀胱中没有检测到。基质细胞类型之间的其他差异表达包括CXC-趋化因子基因的差异表达。CD90+前列腺癌相关基质细胞在多种基因的表达上不同于正常的基质细胞，其中一些基因可能参与器官的发育。
The prostate stroma is a key mediator of epithelial differentiation and development, and potentially plays a role in the initiation and progression of prostate cancer. The tumor-associated stroma is marked by increased expression of CD90/THY1. Isolation and characterization of these stromal cells could provide valuable insight into the biology of the tumor microenvironment. Prostate CD90+ stromal fibromuscular cells from tumor specimens were isolated by cell-sorting and analyzed by DNA microarray. Dataset analysis was used to compare gene expression between histologically normal and tumor-associated stromal cells. For comparison, stromal cells were also isolated and analyzed from the urinary bladder. The tumor-associated stromal cells were found to have decreased expression of genes involved in smooth muscle differentiation, and those detected in prostate but not bladder. Other differential expression between the stromal cell types included that of the CXC-chemokine genes. CD90+ prostate tumor-associated stromal cells differed from their normal counterpart in expression of multiple genes, some of which are potentially involved in organ development.
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影响因子：
6
作者：
Ernst, T;Hergenhahn, M;Gröne, HJ
通讯作者：
Gröne, HJ
影响因子：
6.6
作者：
Liu, AY;Zhang, H;Diamond, DL
通讯作者：
Diamond, DL
DOI：
10.1073/pnas.94.20.10705
发表时间：
1997-09-30
影响因子：
11.1
作者：
Liu, AY;True, LD;vandenEngh, G
通讯作者：
vandenEngh, G
影响因子：
6
作者：
Liu, AY;True, LD
通讯作者：
True, LD
影响因子：
4.8
作者：
Adam, PJ;Boyd, R;Terrett, JA
通讯作者：
Terrett, JA