Expression analysis of α-smooth muscle actin and tenascin-C in the periodontal ligament under orthodontic loading or in vitro culture.
Expression analysis of α-smooth muscle actin and tenascin-C in the periodontal ligament under orthodontic loading or in vitro culture.
复制标题
正畸加载或体外培养牙周膜中α-平滑肌肌动蛋白和腱蛋白-C的表达分析
DOI：
10.1038/ijos.2015.26
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发表时间：
2015-12-18
影响因子：
14.9
通讯作者：
Han, Xiang-Long
中科院分区：
文献类型：
--
作者：
Xu, Hui;Bai, Ding;Ruest, L-Bruno;Feng, Jian Q.;Guo, Yong-Wen;Tian, Ye;Jing, Yan;He, Yao;Han, Xiang-Long
关键词：
α-平滑肌肌动蛋白(α-SMA)和肌张力蛋白C是应激诱导的肌成纤维细胞的表型特征。这两种蛋白的表达水平与细胞外机械微环境密切相关。我们观察了正畸载荷作用下α-SMA和Tenascin-C在牙周膜中的表达变化，以间接揭示牙周膜内在的力学微环境。在这项研究中，我们证实了转化生长因子-β-1(转化生长因子-β-1)和机械拉伸或压缩应力对人牙周膜细胞(HPDLCs)分化为肌成纤维细胞的协同作用。在较高的拉应力和压应力下，hPDLCs的α-SMA和Tenascin-C水平显著高于在较低的拉应力和压应力下的水平。在连续轻或重正畸载荷作用下，大鼠牙周韧带的张力区和压力区也有类似的变化。在表达的时程分析中，我们观察到，在体内正畸负荷下，α-SMA水平的增加与PDL中Tenascin-C水平的增加相匹配。α-SMA和Tenascin-C在PDL中的表达随时间的变化可能反映了恒定外载荷下内应力的随时间变化。
α-smooth muscle actin (α-SMA) and tenascin-C are stress-induced phenotypic features of myofibroblasts. The expression levels of these two proteins closely correlate with the extracellular mechanical microenvironment. We investigated how the expression of α-SMA and tenascin-C was altered in the periodontal ligament (PDL) under orthodontic loading to indirectly reveal the intrinsic mechanical microenvironment in the PDL. In this study, we demonstrated the synergistic effects of transforming growth factor-β1 (TGF-β1) and mechanical tensile or compressive stress on myofibroblast differentiation from human periodontal ligament cells (hPDLCs). The hPDLCs under higher tensile or compressive stress significantly increased their levels of α-SMA and tenascin-C compared with those under lower tensile or compressive stress. A similar trend was observed in the tension and compression areas of the PDL under continuous light or heavy orthodontic load in rats. During the time-course analysis of expression, we observed that an increase in α-SMA levels was matched by an increase in tenascin-C levels in the PDL under orthodontic load in vivo. The time-dependent variation of α-SMA and tenascin-C expression in the PDL may indicate the time-dependent variation of intrinsic stress under constant extrinsic loading.
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循环机械应变后骨髓 MSC 中骨相关基因的表达：对牵张成骨的影响
影响因子：
14.9
作者：
Qi MC;Zou SJ;Han LC;Zhou HX;Hu J
通讯作者：
Hu J
DOI：
10.1083/jcb.200704042
发表时间：
2007-12-17
期刊：
The Journal of cell biology
影响因子：
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作者：
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通讯作者：
Järvinen, M
DOI：
10.1016/s1357-2725(01)00041-3
发表时间：
2001-07-01
影响因子：
4
作者：
Roy, SG;Nozaki, Y;Phan, SH
通讯作者：
Phan, SH
DOI：
10.1016/j.ajodo.2013.05.010
发表时间：
2013-10-01
影响因子：
3
作者：
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通讯作者：
Zou, Shujuan