Development and Characterization of Triticum aestivum-Aegilops longissima 6S(l) Recombinants Harboring a Novel Powdery Mildew Resistance Gene Pm6Sl.
Development and Characterization of Triticum aestivum-Aegilops longissima 6S(l) Recombinants Harboring a Novel Powdery Mildew Resistance Gene Pm6Sl.
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DOI：
10.3389/fpls.2022.918508
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
小麦白粉病是一种在世界范围内传播的叶部病害。培育抗病品种是防治该病最有效、最经济、最环保的策略。抗白粉病基因是抗白粉病育种的主要资源，新的抗白粉病基因的需求不断增加。在此之前，我们鉴定了长山羊草染色体6S1#3为抗白粉病的载体，并将该抗性基因命名为Pm 6S 1。在此，我们报道了基于6S1#3供体Ae的全长cDNA序列设计的24个特异于6S1#3的标记。longissma登录号TA 1910，以及小麦-Ae. longissima 6S1#3渐渗原种通过ph 1b诱导的同源重组。此外，通过白粉病反应的整合分析、原位杂交和分子标记鉴定和表征6S1#3渐渗系，并且将Pm 6S 1定位到6S1#3的长臂中标记Ae 158410和Ae 157699之间42.80Mb的远端间隔。两个抗性重组体R43（T6BS.6BL-6S1#3L）和T27（T16AS.6AL-6S1#3L-6AL）含有携带Pm 6S 1的区段，其具有小于8%的6S1#3基因组长度，并且两个标记可诊断Pm 6S 1。本研究为小麦抗白粉病基因的改良拓宽了基因资源，为进一步研究Pm 6S 1的抗病分子机制提供了精细定位和克隆基础。
Powdery mildew of wheat is a foliar disease that is spread worldwide. Cultivation of resistant varieties is the most effective, economical, and environmentally friendly strategy to curb this disease. Powdery mildew resistance genes (Pm) are the primary resources for resistance breeding, and new Pm genes are in constant demand. Previously, we identified Aegilops longissima chromosome 6Sl#3 as a carrier of powdery mildew resistance and designated the resistance gene as Pm6Sl. Here, we reported the design of 24 markers specific to 6Sl#3 on the basis of the full-length cDNA sequences of 6Sl#3 donor Ae. longissma accession TA1910, and the development of wheat-Ae. longissima 6Sl#3 introgression stocks by ph1b-induced homoeologous recombination. Further, 6Sl#3 introgression lines were identified and characterized by integration analysis of powdery mildew responses, in situ hybridization, and molecular markers and Pm6Sl was mapped to a distal interval of 42.80 Mb between markers Ael58410 and Ael57699 in the long arm of 6Sl#3. Two resistant recombinants, R43 (T6BS.6BL-6Sl#3L) and T27 (Ti6AS.6AL-6Sl#3L-6AL), contained segments harboring Pm6Sl with less than 8% of 6Sl#3 genomic length, and two markers were diagnostic for Pm6Sl. This study broadened powdery mildew resistance gene resources for wheat improvement and provided a fundamental basis for fine mapping and cloning of Pm6Sl to further understand its molecular mechanism of disease resistance.
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