Cell Reservoirs of the Epstein-Barr Virus in Biopsy-proven Lymphocytic Interstitial Pneumonitis in HIV-1 Subtype E Infected Children: Identification by Combined In Situ Hybridization and Immunohistochemistry
Cell Reservoirs of the Epstein-Barr Virus in Biopsy-proven Lymphocytic Interstitial Pneumonitis in HIV-1 Subtype E Infected Children: Identification by Combined In Situ Hybridization and Immunohistochemistry
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HIV-1 E 亚型感染儿童活检证实的淋巴细胞间质性肺炎中 Epstein-Barr 病毒的细胞库：通过原位杂交和免疫组织化学联合鉴定
DOI：
10.1097/pai.0b013e3181baec3a
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发表时间：
2010
影响因子：
1.6
通讯作者：
T. Bhoopat
中科院分区：
文献类型：
--
作者：
L. Bhoopat;Somrak Rangkakulnuwat;Risa Okonogi;K. Wannasai;T. Bhoopat
类风湿性间质性肺炎（LIP）是人类免疫缺陷病毒（HIV）感染儿科患者常见的肺部并发症，其组织学特征为淋巴细胞的显著浸润。有几种理论认为LIP可能是由EB病毒（EBV）引起的。为了确定EB病毒的水库和HIV亚型E感染的儿科LIP淋巴浸润的发病机制，我们检查了9例垂直HIV亚型E感染的儿科患者的手术肺活检证实的LIP中炎性细胞募集中EB病毒的分布和表达。LIP的主要显微镜特征是肺泡间隔广泛增宽，主要由成熟淋巴细胞和浆细胞组成的单核炎性细胞浸润在气道周围并扩展到肺小叶。EB病毒编码的RNA（EBER）原位杂交，从石蜡包埋的肺组织中进行，发现阳性的核内信号在所有9例LIP。有趣的是，在9例LIP中的6例中，联合免疫组织化学和原位杂交分析显示30%至50%的朗格汉斯细胞和相关树突状细胞被EBV感染，而<30%的T和B细胞被EBV感染。提示EBV的慢性抗原刺激在LIP的发病中起重要作用。这支持了郎格罕细胞（LC）更容易感染EBV的观点，表明LC是HIV亚型E感染的儿科LIP肺中EBV的储存库。LC可能在炎症细胞浸润，特别是T细胞进入这些组织中的募集中起重要作用。此外，HIV可能为LIP的进化提供环境或微环境，LIP代表对EBV感染的免疫应答。LC和相关树突状细胞与T细胞之间的相互作用对于有效的HIV和EBV复制是重要的。
Lymphoid interstitial pneumonitis (LIP), a frequent pulmonary complication in human immune deficiency virus (HIV)-infected pediatric patients, is characterized histologically by marked infiltration of lymphoid cells. Several theories have been suggested that LIP may be caused by Epstein-Barr virus (EBV). To identify the reservoir of EBV and pathogenesis of lymphoid infiltrates in HIV subtype E infected pediatric LIP, we examined the distribution and expression of EBV in the inflammatory cell recruitment in surgical lung biopsy-proven LIP from 9 vertically HIV subtype E-infected pediatric patients. The dominant microscopic feature of LIP demonstrated widespread widening of alveolar septum by mononuclear inflammatory cell infiltrate mainly composed of mature lymphocytes and plasma cells surrounding airways and expanding to the lung interstitium. EBV-encoded RNA (EBER) in situ hybridization, performed from paraffin-embedded lung tissues, revealed positive intranuclear signals in all 9 LIP cases. Interestingly, combined immunohistochemical and in situ hybridization analyses in 6 out of 9 LIP cases revealed 30% to 50% of the Langerhans and related dendritic cells were infected with EBV, whereas <30% of the T and B cells were infected with EBV. These results suggested that a chronic antigenic stimulus of EBV played important roles in the pathogenesis of LIP in these patients. This supports the notion that Langerhans cells (LCs) are more readily infected with EBV, indicating that LCs are reservoirs for EBV in lungs of HIV subtype E-infected pediatric LIP. And possibly LCs may play an important role in the recruitment of inflammatory cell infiltrates, especially T cells into these tissues. In addition, HIV may provide a milieu or microenvironment for the evolution of LIP, which represent an immunologic response to EBV infection. Interactions between LCs and related dendritic cells together with T cells are important for effective HIV and EBV replications.
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DOI：
10.1016/s0022-3476(05)80432-9
发表时间：
1992
期刊：
The Journal of pediatrics
影响因子：
--
作者：
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通讯作者：
Shapiro,ED
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DOI：
10.1086/378072
发表时间：
2003
期刊：
The Journal of infectious diseases.
影响因子：
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作者：
Walling,DennisM;Flaitz,CatherineM;Nichols,CMark
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Nichols,CMark
肺淋巴增殖性疾病中的寡克隆 T 细胞扩增：证明寡克隆 T 细胞在人类免疫缺陷病毒相关淋巴样间质性肺炎中频繁发生。
DOI：
10.1164/ajrccm.165.2.2101141
发表时间：
2002
期刊：
American journal of respiratory and critical care medicine.
影响因子：
--
作者：
Kurosu,Katsushi;Yumoto,Norio;Rom,WilliamN;Takiguchi,Yuichi;Jaishree,Jagirdai;Nakata,Koh;Tatsumi,Koichiro;Mikata,Aatsuo;Kuriyama,Takatyuki;Weiden,MichaelD
通讯作者：
Weiden,MichaelD
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DOI：
10.1086/422039
发表时间：
2004
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Walling,DennisM;Ling,PaulD;Gordadze,AlexeyV;Montes-Walters,Miguel;Flaitz,CatherineM;Nichols,CMark
通讯作者：
Nichols,CMark
儿童淋巴样间质性肺炎和低丙种球蛋白血症。
DOI：
10.1164/arrd.1981.124.4.491
发表时间：
1981
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Church,JA;Isaacs,H;Saxon,A;Keens,TG;Richards,W
通讯作者：
Richards,W