Immunohistochemical studies of the peritoneal membrane and infiltrating cells in normal subjects and in patients on CAPD.
Immunohistochemical studies of the peritoneal membrane and infiltrating cells in normal subjects and in patients on CAPD.
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对正常受试者和 CAPD 患者的腹膜和浸润细胞进行免疫组织化学研究。
DOI：
10.1038/ki.1994.292
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发表时间：
1994
影响因子：
19.6
通讯作者：
J. S. Cameron
中科院分区：
文献类型：
--
作者：
J. Suassuna;J. Suassuna;Fernando C. Das Neves;Fernando C. Das Neves;R. B. Hartley;R. B. Hartley;Chisholm S. Ogg;Chisholm S. Ogg;J. S. Cameron;J. S. Cameron
我们对33名受试者的壁腹膜活检进行了免疫组织化学研究，以调查在CAPD期间，除了透析液中游离的单个核细胞外，其他细胞群体是否可能参与腹膜防御微生物的入侵。白细胞集中在两个区域：一层是由显示激活和成熟标志的细长巨噬细胞组成的间皮下层，另一层是血管周围与T细胞密切相关的成熟程度较低的巨噬细胞，以及表达HLA-DR、ICAM-1和VCAM-1的内皮细胞。正常间皮细胞表达转铁蛋白受体和黏附分子ICAM-1和VCAM-1，但不表达ELAM-1。正常组和尿毒症组之间无显著差异，而腹膜透析组表现为轻度间皮下层紊乱和内皮细胞表面HLA-DR表达轻微上调。腹膜炎与腹膜间皮下细胞增多有关，尤其是血管周围白细胞的浸润，并伴随着HLA-DR和黏附分子的表达增加。除了透析液中游离的单个核细胞外，本研究还证明腹膜存在两个额外的白细胞群：腹膜间皮下巨噬细胞、血管周围巨噬细胞和T细胞。这也提示存在第四种黏附于间皮细胞的腔内白细胞。现在有必要进行研究，以评估它们在CAPD期间预防腹膜炎的宿主防御中的确切作用。
We performed immunohistochemical studies on biopsies of the parietal peritoneal membrane of 33 subjects to investigate whether other cell populations, in addition to mononuclear cells free in the dialysate, might participate in the defense of the peritoneum against microbial invasion during CAPD. Leukocytes were found to concentrate in two areas: a submesothelial layer composed of elongated macrophages displaying activation and maturation markers, and perivascular, less mature macrophages closely associated with T cells and HLA-DR, ICAM-1 and VCAM-1 expressing endothelial cells. Normal mesothelial cells were found to express constitutively the transferrin receptor and the adhesion molecules ICAM-1 and VCAM-1 but not ELAM-1. There were no major differences between normal and uremic subjects, while peritoneal dialysis patients exhibited minor derangements of the submesothelial layer and slight up-regulation of the expression of HLA-DR on endothelial cells. Peritonitis was associated with increased submesothelial cellularity and, particularly, perivascular leukocyte infiltration accompanied by increased expression of HLA-DR and adhesion molecules. Besides mononuclear cells free in the dialysate, this study demonstrates the existence of two additional peritoneal membrane leukocyte populations: submesothelial macrophages, and perivascular macrophages and T cells. It also suggests the existence of a fourth population of intracavitary leukocytes adherent to mesothelial cells. Studies are now necessary to evaluate their exact role in the host defence against peritonitis during CAPD.
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DOI：
--
发表时间：
1991
期刊：
The American journal of pathology
影响因子：
--
作者：
G. Rice;J. Munro;Christopher L. Corless;M. Bevilacqua
通讯作者：
G. Rice;J. Munro;Christopher L. Corless;M. Bevilacqua
DOI：
--
发表时间：
1988-12
期刊：
The American journal of pathology
影响因子：
--
作者：
J. Pober
通讯作者：
J. Pober
影响因子：
4.4
作者：
Michael Loran Dustin;R. Rothlein;A. Bhan;C. Dinarello;T. Springer
通讯作者：
Michael Loran Dustin;R. Rothlein;A. Bhan;C. Dinarello;T. Springer
通过内侧基底下丘脑的喙部传入神经阻滞逆转 delta-9-四氢大麻酚对催乳素分泌的抑制作用。
影响因子：
4.1
作者：
Tyrey,L
通讯作者：
Tyrey,L
血管周围树突状巨噬细胞作为人真皮微血管单元的免疫生物学成分。
DOI：
10.1111/1523-1747.ep12581078
发表时间：
1989
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Sontheimer,RD
通讯作者：
Sontheimer,RD