Expression Pattern and Functional Analyses of Arabidopsis Guard Cell-Enriched GDSL Lipases.
Expression Pattern and Functional Analyses of Arabidopsis Guard Cell-Enriched GDSL Lipases.
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DOI：
10.3389/fpls.2021.748543
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发表时间：
2021
影响因子：
5.6
通讯作者：
Hu H
中科院分区：
文献类型：
--
作者：
Xiao C;Guo H;Tang J;Li J;Yao X;Hu H
拟南芥中有 100 多种 GDSL 脂肪酶，但只有少数成员进行了功能研究。此外，还没有报道对气孔生物学中的 GDSL 进行全面分析。在这里，我们系统地研究了 19 种假定的富含警卫细胞的 GDSL 脂肪酶 (GGL) 在不同发育阶段以及对激素和非生物胁迫处理的反应的表达模式。基因表达分析表明这些 GGL 具有不同的表达模式。 15 个 GGL 在保卫细胞中高表达，其中 7 个优先在保卫细胞中表达。大多数GGL定位于内质网，也有一些定位于脂滴和细胞核。一些密切同源的GGL在不同组织中表现出相似的表达模式，并对激素和非生物胁迫作出反应，或相似的亚细胞定位，表明表达模式与生物功能的相关性，以及GGL在植物发育和环境适应中的功能冗余。 ggl突变体的进一步表型鉴定表明，GGL7、GGL14、GGL22和GGL26在气孔动力学、气孔密度和形态以及植物水分关系中发挥独特且冗余的作用。本研究为深入了解这些 GGL 的功能提供了独特的资源，以控制气孔动态和发育、植物生长和对环境的适应。
There are more than 100 GDSL lipases in Arabidopsis, but only a few members have been functionally investigated. Moreover, no reports have ever given a comprehensive analysis of GDSLs in stomatal biology. Here, we systematically investigated the expression patterns of 19 putative Guard-cell-enriched GDSL Lipases (GGLs) at various developmental stages and in response to hormone and abiotic stress treatments. Gene expression analyses showed that these GGLs had diverse expression patterns. Fifteen GGLs were highly expressed in guard cells, with seven preferentially in guard cells. Most GGLs were localized in endoplasmic reticulum, and some were also localized in lipid droplets and nucleus. Some closely homologous GGLs exhibited similar expression patterns at various tissues and in response to hormone and abiotic stresses, or similar subcellular localization, suggesting the correlation of expression pattern and biological function, and the functional redundancy of GGLs in plant development and environmental adaptations. Further phenotypic identification of ggl mutants revealed that GGL7, GGL14, GGL22, and GGL26 played unique and redundant roles in stomatal dynamics, stomatal density and morphology, and plant water relation. The present study provides unique resources for functional insights into these GGLs to control stomatal dynamics and development, plant growth, and adaptation to the environment.
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