Inflammation and enhanced atherogenesis in the carotid artery with altered blood flow in an atherosclerosis-resistant mouse strain.
Inflammation and enhanced atherogenesis in the carotid artery with altered blood flow in an atherosclerosis-resistant mouse strain.
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DOI：
10.14814/phy2.14829
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发表时间：
2021-06
影响因子：
2.5
通讯作者：
Shi W
中科院分区：
文献类型：
--
作者：
Zhao J;Huangfu C;Chang Z;Zhou W;Grainger AT;Liu Z;Shi W
载脂蛋白E-缺陷（Apoe-/-）小鼠颈总动脉分叉附近的结扎导致动脉粥样硬化的快速发展，这受到遗传背景的影响。BALB/cJ（BALB）小鼠对动脉粥样硬化有抵抗力，其主动脉病变比C57 BL/6（B6）小鼠小得多。在这项研究中，我们检查了导致B6和BALB Apoe−/−小鼠颈动脉病变形成的细胞事件，无论是否有血流限制。一组小鼠结扎左侧颈总动脉分叉处，阻断血流，另一组不进行手术干预。在不中断血流的情况下，BALB‐Apoe−/−小鼠在12周的西方饮食后形成的动脉粥样硬化病变比B6‐Apoe−/−小鼠小得多（3，325 ± 1，086 vs. 81，549 ± 9，983 µm2/切片; p = 2.1E‐7）。病变发生在两个菌株的动脉分叉。当血流受阻时，两种品系的结扎颈动脉均显示出显著的脂质沉积、炎性细胞浸润和快速斑块形成。BALB小鼠结扎后3天和B6小鼠结扎后1周动脉壁内可见中性粒细胞和巨噬细胞。在BALB小鼠内膜病变中观察到CD 4 T细胞，而在B6小鼠内膜病变中未观察到CD 4 T细胞。到4周时，两种菌株都出现了类似大小的晚期病变，包括泡沫细胞、平滑肌细胞和新生血管。动脉粥样硬化也发生在对侧颈总动脉的直线区域，其中MCP-1在BALB小鼠的内膜中大量表达。这些结果表明，在高脂血症的BALB小鼠中，血流紊乱比高脂饮食更显著地促进炎症和动脉粥样硬化。我们研究了C57 BL/6（B6）和BALB/c（BALB）Apoe−/−小鼠品系在有或没有血流限制的情况下导致颈动脉病变形成的细胞事件。在没有血流中断的情况下，BALB‐Apoe−/−小鼠在西方饮食12周后形成的动脉粥样硬化病变比B6‐Apoe−/−小鼠小得多。当血流受阻时，两种品系的结扎颈动脉均显示出明显的脂质沉积、炎性细胞浸润和快速斑块形成。
Ligation of the common carotid artery near its bifurcation in apolipoprotein E‐deficient (Apoe−/−) mice leads to rapid atherosclerosis development, which is affected by genetic backgrounds. BALB/cJ (BALB) mice are resistant to atherosclerosis, developing much smaller aortic lesions than C57BL/6 (B6) mice. In this study, we examined cellular events leading to lesion formation in carotid arteries with or without blood flow restriction of B6 and BALB Apoe−/− mice. Blood flow was obstructed by ligating the left common carotid artery near its bifurcation in one group of mice, and other group received no surgical intervention. Without blood flow interruption, BALB‐Apoe−/− mice formed much smaller atherosclerotic lesions than B6‐Apoe−/− mice after 12 weeks of Western diet (3,325 ± 1,086 vs. 81,549 ± 9,983 µm2/section; p = 2.1E‐7). Lesions occurred at arterial bifurcations in both strains. When blood flow was obstructed, ligated carotid artery of both strains showed notable lipid deposition, inflammatory cell infiltration, and rapid plaque formation. Neutrophils and macrophages were observed in the arterial wall of BALB mice 3 days after ligation and 1 week after ligation in B6 mice. CD4 T cells were observed in intimal lesions of BALB but not B6 mice. By 4 weeks, both strains developed similar sizes of advanced lesions containing foam cells, smooth muscle cells, and neovessels. Atherosclerosis also occurred in straight regions of the contralateral common carotid artery where MCP‐1 was abundantly expressed in the intima of BALB mice. These findings indicate that the disturbed blood flow is more prominent than high fat diet in promoting inflammation and atherosclerosis in hyperlipidemic BALB mice. We examined cellular events leading to lesion formation in carotid arteries with or without blood flow restriction of C57BL/6 (B6) and BALB/c (BALB) Apoe−/− mouse strains. Without blood flow interruption, BALB‐Apoe−/− mice formed much smaller atherosclerotic lesions than B6‐Apoe−/− mice after 12 weeks of Western diet. When blood flow was obstructed, the ligated carotid artery of both strains showed notable lipid deposition, inflammatory cell infiltration and rapid plaque formation.
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影响因子：
6.5
作者：
Brown, MD;Jin, L;Shi, WB
通讯作者：
Shi, WB
影响因子：
5.3
作者：
Eschert, H.;Sindermann, J. R.;Weissen-Plenz, G.
通讯作者：
Weissen-Plenz, G.
DOI：
10.1161/01.atv.17.11.2333
发表时间：
1997-11-01
影响因子：
8.7
作者：
Bonthu, S;Heistad, DD;Faraci, FM
通讯作者：
Faraci, FM
DOI：
10.1161/01.atv.17.10.2238
发表时间：
1997-10-01
影响因子：
8.7
作者：
Kumar, A;Lindner, V
通讯作者：
Lindner, V
DOI：
10.1161/01.atv.14.1.133
发表时间：
1994-01-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
NAKASHIMA, Y;PLUMP, AS;ROSS, R
通讯作者：
ROSS, R