Liguleless narrow and narrow odd dwarf act in overlapping pathways to regulate maize development and metabolism.
Liguleless narrow and narrow odd dwarf act in overlapping pathways to regulate maize development and metabolism.
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DOI：
10.1111/tpj.15988
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发表时间：
2022-11
期刊：
影响因子：
7.2
通讯作者：
Brunkard, Jacob O.
中科院分区：
文献类型：
--
作者：
Jazmin Abraham-Juarez, Maria;Busche, Michael;Anderson, Alyssa A.;Lunde, China;Winders, Jeremy;Christensen, Shawn A.;Hunter, Charles T.;Hake, Sarah;Brunkard, Jacob O.
关键词：
窄奇矮（nod）和无叶舌窄（Lgn）是多效性玉米突变体，都编码质膜蛋白，导致类似的发育模式缺陷，并组成性诱导应激信号通路。为了研究这些突变体如何协调玉米的发育和生理，我们通过亲和纯化筛选NOD的蛋白质相互作用。LGN被确定为一个强的候选相互作用，我们通过正交实验证实了NOD-LGN分子相互作用。我们进一步证明了LGN，一种受体样激酶，可以在体外磷酸化NOD，暗示它们可以在交叉信号转导通路中起作用。为了验证这一假设，我们在两种背景（B73和A619）中产生了Lgn-R;nod突变体，并发现这些突变相互增强，导致比任何单一突变本身更严重的发育缺陷，表型包括非常狭窄的叶子，分蘖增加和主枝失败。转录组学和代谢组学分析的单和双突变体在两个遗传背景揭示了广泛的诱导病原体防御基因和资源分配的转变，从初级代谢有利于专门的代谢。这些影响在每个单突变体中是相似的，在双突变体中是增强的，这使我们得出结论，NOD和LGN在重叠的信号通路中累积作用，以协调玉米的生长-防御权衡。
Narrow odd dwarf (nod) and Liguleless narrow (Lgn) are pleiotropic maize mutants that both encode plasma membrane proteins, cause similar developmental patterning defects, and constitutively induce stress signaling pathways. To investigate how these mutants coordinate maize development and physiology, we screened for protein interactors of NOD by affinity purification. LGN was identified by this screen as a strong candidate interactor, and we confirmed the NOD-LGN molecular interaction through orthogonal experiments. We further demonstrated that LGN, a receptor-like kinase, can phosphorylate NOD in vitro, hinting that they could act in intersecting signal transduction pathways. To test this hypothesis, we generated Lgn-R;nod mutants in two backgrounds (B73 and A619), and found that these mutations enhance each other, causing more severe developmental defects than either single mutation on its own, with phenotypes including very narrow leaves, increased tillering, and failure of the main shoot. Transcriptomic and metabolomic analyses of the single and double mutants in the two genetic backgrounds revealed widespread induction of pathogen defense genes and a shift in resource allocation away from primary metabolism in favor of specialized metabolism. These effects were similar in each single mutant and heightened in the double mutant, leading us to conclude that NOD and LGN act cumulatively in overlapping signaling pathways to coordinate growth-defense tradeoffs in maize.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
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通讯作者：
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影响因子：
30.8
作者：
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通讯作者：
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影响因子：
7.2
作者：
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Buckler, ES
影响因子：
11.6
作者：
Bolduc, Nathalie;Hake, Sarah
通讯作者：
Hake, Sarah
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影响因子：
64.5
作者：
Bi, Guozhi;Su, Min;Zhou, Jian-Min
通讯作者：
Zhou, Jian-Min