The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization.
The Use of a Replicating Virus Vector For in Planta Generation of Tobacco Mosaic Virus Nanorods Suitable For Metallization.
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DOI：
10.3389/fbioe.2022.877361
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发表时间：
2022
影响因子：
5.7
通讯作者：
Lomonossoff, George P.
中科院分区：
文献类型：
--
作者：
Saunders, Keith;Thuenemann, Eva C.;Shah, Sachin N.;Peyret, Hadrien;Kristianingsih, Ruth;Lopez, Sergio G.;Richardson, Jake;Lomonossoff, George P.
关键词：
在植物中生产设计长度烟草花叶病毒（TMV）纳米棒在产量方面一直存在问题，特别是在加入修饰的外壳蛋白亚基时。为了解决这个问题，我们研究了使用复制马铃薯病毒x基载体（pEff）来表达含有野生型或修饰版TMV外壳蛋白的定义长度的纳米棒。该系统先前已被证明是一种有效的方法，以产生丝状植物病毒的病毒样颗粒。所得到的TMV纳米棒的长度可以通过改变封装RNA的长度来控制。使用自定义编写的Python计算机脚本以及纳米棒UI用户界面脚本分析纳米棒长度，从而生成粒子长度的直方图。此外，纳米棒变体是通过结合在其c端呈现金属结合肽的外壳蛋白亚基产生的。我们通过生成结合胶体金纳米颗粒的纳米棒来证明这种方法的实用性。
The production of designer-length tobacco mosaic virus (TMV) nanorods in plants has been problematic in terms of yields, particularly when modified coat protein subunits are incorporated. To address this, we have investigated the use of a replicating potato virus X-based vector (pEff) to express defined length nanorods containing either wild-type or modified versions of the TMV coat protein. This system has previously been shown to be an efficient method for producing virus-like particles of filamentous plant viruses. The length of the resulting TMV nanorods can be controlled by varying the length of the encapsidated RNA. Nanorod lengths were analyzed with a custom-written Python computer script coupled with the Nanorod UI user interface script, thereby generating histograms of particle length. In addition, nanorod variants were produced by incorporating coat protein subunits presenting metal-binding peptides at their C-termini. We demonstrate the utility of this approach by generating nanorods that bind colloidal gold nanoparticles.
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影响因子：
3
作者：
Avesani, Linda;Marconi, Giampiero;Porceddu, Andrea
通讯作者：
Porceddu, Andrea
DOI：
10.1073/pnas.79.19.5818
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GOELET, P;LOMONOSSOFF, GP;KARN, J
通讯作者：
KARN, J
影响因子：
13.8
作者：
Sainsbury, Frank;Thuenemann, Eva C.;Lomonossoff, George P.
通讯作者：
Lomonossoff, George P.
影响因子：
--
作者：
Bromley, Keith M.;Patil, Avinash J.;Mann, Stephen
通讯作者：
Mann, Stephen
影响因子：
10.8
作者：
Huang, Y;Chiang, CY;Belcher, AM
通讯作者：
Belcher, AM