Volatiles from soil-borne fungi affect directional growth of roots.
Volatiles from soil-borne fungi affect directional growth of roots.
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DOI：
10.1111/pce.13890
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Cordovez V
中科院分区：
文献类型：
--
作者：
Moisan K;Raaijmakers JM;Dicke M;Lucas-Barbosa D;Cordovez V
挥发物在土壤（微生物）有机体和植物之间的生态相互作用中起着重要作用。众所周知，微生物挥发物可以增加根系生物量和侧根形成。然而，到目前为止，微生物挥发物是否会影响定向根生长还不清楚。在这里，我们提出了一种研究地下挥发物介导的相互作用的新方法。作为概念验证，我们设计了一个Y型根管嗅觉仪，并测试了四种不同土传真菌的挥发物对芜菁根在土壤中定向生长的影响。随后，我们比较了真菌挥发性有机化合物（VOCs）与气相色谱-质谱法（GC-MS）的轮廓。使用我们新设计的设置，我们表明，在土壤中的定向根生长的差异真菌挥发物的影响。根生长更频繁地朝向挥发物从根病原体立枯丝核菌，而挥发物从其他三个腐殖真菌没有影响定向根生长。GC-MS分析表明，6种挥发性有机物都是由R.索拉尼这些研究结果证实，这种新方法适合于揭示根系和土传真菌之间有趣的化学串扰及其对根系生长的影响。
Volatiles play major roles in mediating ecological interactions between soil (micro)organisms and plants. It is well‐established that microbial volatiles can increase root biomass and lateral root formation. To date, however, it is unknown whether microbial volatiles can affect directional root growth. Here, we present a novel method to study belowground volatile‐mediated interactions. As proof‐of‐concept, we designed a root Y‐tube olfactometer, and tested the effects of volatiles from four different soil‐borne fungi on directional growth of Brassica rapa roots in soil. Subsequently, we compared the fungal volatile organic compounds (VOCs) previously profiled with Gas Chromatography–Mass Spectrometry (GC–MS). Using our newly designed setup, we show that directional root growth in soil is differentially affected by fungal volatiles. Roots grew more frequently toward volatiles from the root pathogen Rhizoctonia solani, whereas volatiles from the other three saprophytic fungi did not impact directional root growth. GC–MS profiling showed that six VOCs were exclusively emitted by R. solani. These findings verify that this novel method is suitable to unravel the intriguing chemical cross‐talk between roots and soil‐borne fungi and its impact on root growth.
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