Histotripsy Ablation of Bone Tumors: Feasibility Study in Excised Canine Osteosarcoma Tumors.
Histotripsy Ablation of Bone Tumors: Feasibility Study in Excised Canine Osteosarcoma Tumors.
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DOI：
10.1016/j.ultrasmedbio.2021.08.004
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发表时间：
2021-12
影响因子：
2.9
通讯作者：
Vlaisavljevich E
中科院分区：
文献类型：
--
作者：
Arnold L;Hendricks-Wenger A;Coutermarsh-Ott S;Gannon J;Hay AN;Dervisis N;Klahn S;Allen IC;Tuohy J;Vlaisavljevich E
骨肉瘤（OS）是一种影响狗和人类的原发性骨肿瘤。组织解剖学是一种非热、非侵入性聚焦超声方法，利用受控声空化来机械分解组织。在这项研究中，我们研究了使用 500 kHz 换能器对弗吉尼亚理工大学兽医教学医院手术后采集的切除犬 OS 样本进行组织解剖学治疗原发性 OS 肿瘤的可行性。将样品包埋在明胶组织模型中，并使用 500 kHz 组织解剖系统进行处理，使用 1-2 个周期脉冲，脉冲重复频率为 250 Hz，剂量为 4,000 个脉冲/点。单独的实验还使用相同的脉冲参数测试了对正常犬骨骼和神经的组织解剖效果。治疗后，完成样本的组织病理学评估。为了证明通过完整皮肤/软组织治疗 OS 的可行性，额外的组织解剖实验用覆盖的组织测试了 OS。在 26.2 ± 4.5 MPa 的峰值负压下，所有肿瘤样品的焦点处均实现了气泡云的生成。组织病理学显示 OS 肿瘤治疗区域的细胞有效消融，正常组织中没有细胞死亡或组织损伤的证据。通过组织/皮肤的治疗导致在操作系统肿瘤内产生良好限制的气泡云和消融区域。结果证明了组织解剖学治疗 OS 肿瘤的可行性。
Osteosarcoma (OS) is a primary bone tumor affecting both dogs and humans. Histotripsy is a non-thermal, non-invasive focused ultrasound method using controlled acoustic cavitation to mechanically disintegrate tissue. In this study, we investigate the feasibility of treating primary OS tumors with histotripsy using a 500 kHz transducer on excised canine OS samples harvested after surgery at the Veterinary Teaching Hospital at Virginia Tech. Samples were embedded in gelatin tissue phantoms and treated with the 500 kHz histotripsy system using 1–2 cycle pulses at a pulse repetition frequency of 250 Hz and a dosage of 4,000 pulses/point. Separate experiments also tested histotripsy effects on normal canine bone and nerve using the same pulsing parameters. After treatment, histopathological evaluation of the samples was completed. To demonstrate the feasibility of treating OS through intact skin/soft tissue, additional histotripsy experiments tested OS with overlying tissues. Generation of bubble clouds was achieved at the focus in all tumor samples at peak negative pressures of 26.2 ± 4.5 MPa. Histopathology showed effective cell ablation in treated areas for OS tumors, with no evidence of cell death or tissue damage in normal tissues. Treatment through tissue/skin resulted in generation of well-confined bubble clouds and ablation zones inside OS tumors. Results demonstrate the feasibility of treating OS tumors with histotripsy.
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DOI：
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发表时间：
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影响因子：
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发表时间：
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影响因子：
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DOI：
10.1186/s13046-020-01685-w
发表时间：
2020-09-04
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
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